阳和汤药组配伍对不同期Lewis肺癌荷瘤小鼠的抑瘤效应及Th1/Th2漂移的影响  

The Anti-tumor Effect and Th1/Th2 Drifting of Yanghe Tang Drugs on Lewis Lung Cancer Tumor Bearing Mice at Different Ages

作　　者：叶峥嵘[1] 吴琳 YE Zheng-rong;WU Lin(Shaanxi University of Chinese Medicine,Xianyang 712046,China;Xianyang Central Hospital,Xianyang 712000,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]咸阳市中心医院,陕西咸阳712000

出　　处：《江西中医药大学学报》2020年第5期89-93,共5页Journal of Jiangxi University of Chinese Medicine

基　　金：陕西省科技厅社会发展项目(2018SF-303);陕西省教育厅科学研究项目(17JK0204);陕西省中医管理局中医药科研项目(JCMS073);咸阳市科学技术研究计划项目(2017k02-93)。

摘　　要：目的:探讨阳和汤药组配伍对不同期Lewis肺癌荷瘤小鼠的抑瘤效应及Th1/Th2漂移的影响。方法:先后以相同的方法建立荷瘤10天和5天的Lewis肺癌荷瘤小鼠动物模型,分别用阳和汤温补肾阳药组和阳和汤温阳散寒药组及阳和汤两药组合用水煎液连续灌胃给药10天,观察不同期各组Lewis肺癌荷瘤小鼠的体重、脾指数、胸腺指数、抑瘤效应及Th1/Th2漂移的血清标志性细胞因子IL-2和IL-4含量。结果:在荷瘤5天的Lewis肺癌荷瘤小鼠中,与模型对照组比较,阳和汤温阳散寒药组和阳和汤两药组合用有明显的抑瘤效应且血清IL-4含量降低(P<0.01),阳和汤温阳散寒药组荷瘤小鼠胸腺指数降低且血清IL-2含量升高(P<0.05),阳和汤两药组合用荷瘤小鼠血清IL-2含量明显升高(P<0.01)。在荷瘤10天的Lewis肺癌荷瘤小鼠中,与模型对照组比较,阳和汤温阳散寒药组和阳和汤温补肾阳药组及阳和汤两药组合用均具有抑瘤效应并降低荷瘤小鼠血清IL-4含量(P<0.05),其中阳和汤两药组合用的作用更明显(P<0.01);阳和汤温补肾阳药组和阳和汤两药组合用组的荷瘤小鼠体重、脾指数和胸腺指数升高(P<0.05),且血清IL-2的含量明显升高(P<0.01)。结论:阳和汤温阳散寒药组和阳和汤两药组合用对荷瘤5天的Lewis肺癌荷瘤小鼠的移植瘤具有明显的抑制作用,该作用机制可能与提高Th1细胞因子IL-2有关,但更与降低Th2细胞因子IL-4含量关系密切。阳和汤温阳散寒药组和阳和汤温补肾阳药组及阳和汤两药组合用对荷瘤10天的Lewis肺癌荷瘤小鼠的移植瘤均具有抑制作用,且阳和汤两药组合用更明显。该作用机制可能与降低Th2细胞因子IL-4含量有关。而温补肾阳药组和阳和汤两药组合用的作用机制还可能与提高荷瘤小鼠体重、脾指数和胸腺指数及Th1细胞因子IL-2含量关系密切。Objective:To discuss the anti-tumor effect and Th1/Th2 drifting of Yanghe Tang drugs on Lewis lung cancer tumor bearing mice at different ages.Methods:Establishing a 10-day and a 5-day Lewis lung cancer tumor bearing mice model in the same way.The mice were intragastric administration with Yanghe Tang Wenbu Shenyang drugs,Yanghe Tang Wenyang Sanhan drugs and two combined drugs for 10 days.Then the mice in different groups which were of different ages were observed weight,spleen index,thymus index,anti-tumor effect and IL2,IL4 level in serum.Results:When the fifth day of mice transplanted tumor,compared with model control group,mice tumors were significantly inhibited and IL4 content in serum was decreased which in Yanghe Tang Wenyang Sanhan drugs group and two combined drugs group(P<0.01).The thymus index of Yanghe Tang Wenyang Sanhan drugs group mice was decreased,while IL2 level in serum of mice was increased(P<0.05).IL2 level of two combined drugs group mice was significantly increased(P<0.01).When the tenth day of mice transplanted tumor,compared with model control group,mice tumors were inhibited and IL4 level in serum was decreased which in Yanghe Tang Wenbu Shenyang,Yanghe Tang Wenyang Sanhan and two combined drugs groups(P<0.05),the effect in two combined drugs group was more obvious(P<0.01).The mice weight,spleen index,thymus index were all elevated of Yanghe Tang Wenbu Shenyang and two combined drugs groups(P<0.05),and IL2 level in serum was significantly increased(P<0.01).Conclusion:The transplantation tumor in Lewis lung cancer tumor bearing mice was obvious inhibited by both Yanghe Tang Wenyang Sanhan drug and two combined drugs when the mice were transplanted tumor 5 days.The mechanism may be related to improve the IL2 level,but it is much close to reduce the IL4 content.All of Yanghe Tang Wenyang Sanhan drug,Yanghe Tang Wenbu Shenyang drug and two combined drugs can inhibit transplantation tumor when Lewis lung cancer tumor bearing mice were transplanted tumor for 10 days,and the effect of two comb

关 键 词：阳和汤 温补肾阳 温阳散寒 LEWIS肺癌荷瘤小鼠 TH1/TH2漂移 

分 类 号：R285.5[医药卫生—中药学]