栽培茄(Solanum melongena L.)与喀西茄(Solanum khasianum C.B.Clarke)F1杂交种的获得与鉴定  

Obtain and Identification of F1 hybrid of Solanum melongena L.and Solanum khasianum C.B.Clarke

作　　者：薛金燕 刘益勇 吴隽香 徐照璨 张宇[1] 成玉富[1] 杨旭[1] XUE Jin-yan;LIU Yi-yong;WU Jun-xiang;XU Zhao-can;ZHANG Yu;CHENG Yu-fu;YANG Xu(School of Horticulture and Plant Protection,Yangzhou University,Agriculture and Agricultural Product Safety International Cooperation Joint Laboratory,Yangzhou University,Ministry of Education,Yangzhou 225009,Jiangsu,China)

机构地区：[1]扬州大学园艺与植物保护学院,扬州大学教育部农业与农产品安全国际合作联合实验室,江苏扬州225009

出　　处：《中国蔬菜》2020年第10期62-67,共6页China Vegetables

基　　金：国家自然科学基金项目(31972395);扬州大学研究生科研创新项目(NXKYCX19-112)。

摘　　要：喀西茄(Solanum khasianum C.B.Clarke)是茄子近缘野生种,具有珍贵的抵抗生物胁迫及非生物胁迫的基因。以10份栽培茄材料作母本与喀西茄进行杂交,并采用一次授粉、重复授粉、花柱短截、混合授粉及嫁接后授粉等授粉方式,研究获得栽培茄与喀西茄种间杂交种的最佳方法。结果表明:采用嫁接后授粉可以提高杂交结果率,并且得到了L81与喀西茄的F1;对F1苗期植株进行SSR分子鉴定,证实了F1种子为栽培茄与喀西茄种间杂交种。Solanum khasianum C.B.Clarke is an affinis wild eggplant species,possessing valuable genes resisting both biotic stress and abiotic stress.Taking 10 cultivated eggplants as female parent,proceeding hybridization with Solanum khasianum C.B.Clarke,and adopting pollination methods as once pollination,repeating pollination,style cutting back,mixture pollination and pollination after grafting,etc.,this study had obtained the best way for interspecies hybridizatyon between Solanum melongena L.and Solanum khasianum C.B.Clarke.The results showed that adopting pollination after grafting could increase hybrid fruit setting rate,and obtain F1 hybrid between L81 and Solanum khasianum C.B.Clarke.SSR molecular identification was conducted on F1 plant at seedling stage and the F1 seed was proven to be the seed of interspecific hybridization between Solanum melongena L.and Solanum khasianum C.B.Clarke.

关 键 词：喀西茄 远缘杂交 嫁接 SSR 

分 类 号：S641.1[农业科学—蔬菜学]