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作 者:顾玉阳 王黎娜 袁娟 张艺 姚陆铭[1] 马晓红[1] 王彪[1] 武天龙[1] GU Yu-yang;WANG Li-na;YUAN Juan;ZHANG Yi;YAO Lu-ming;MA Xiao-hong;WANG Biao;WU Tian-long(College of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Shanghai Honggang Green Hyacinth Bean Production Professional Cooperation,Shanghai 201306,China;Shanghai Pudong Agricultural Technology Extension Center,Shanghai 201201,China)
机构地区:[1]上海交通大学农业与生物学院,上海200240 [2]上海红刚青扁豆生产专业合作社,上海201306 [3]上海市浦东农业技术推广中心,上海201201
出 处:《中国蔬菜》2020年第10期68-76,共9页China Vegetables
基 金:上海市科技兴农重点攻关项目〔沪农科创字(2018)第2-4号〕。
摘 要:近几年,在上海浦东扁豆主产区暴发了一种病害类似于其他作物上发生的菌核病。通过对分离纯化的病原菌进行形态学观察、致病性鉴定以及分子生物学特征分析,确定了该致病菌就是核盘菌属的核盘菌〔Sclerotinia sclerotiorum(Lib.)de Bary〕,该菌可以感染扁豆全株,包括根、茎、叶、花、果实都能感染发病,并且能观察到菌核的形成。研究发现扁豆核盘菌室内分离培养的最佳碳源是果糖,温度22℃,pH为5,适当的逆境生长条件有利于菌核的形成。扁豆、大豆、普通白菜来源的核盘菌在ITS rDNA序列上存在差异。将3种不同来源的核盘菌相比较,发现它们属于不同的融合群,对不同寄主存在致病能力上的差异,但都能致病。In recent years,a sclerotinia disease similar to symptoms of the other crops has broken out at the main hyacinth bean producing area in Shanghai Pudong district.Through conducting mophological observation,pathogenicity identification on separated and purified pathogenic bacteria,analyzing molecular biology characteristics,we confirmed that the pathogen causing this disease was Sclerotinia sclerotiorum(Lib.)de Bary.This pathogen could infect the whole plant of hyacinth bean,including its root,stem,leaf,flower and pod.We had also observed the formation of its sclerotia.This study discovered that the best growth carbon source for indoor seperation and culture of Sclerotinia sclerotiorum was fructose at 22℃,pH 5.Proper growth conditions under stress were in favour of forming sclerotia.There were differences existed in ITS rDNA sequences of Sclerotinia sclerotiorum from hyacinth bean,soybean and pakchoi.Comparing Sclerotinia sclerotiorum originating from those different sources,we found that those strains belonged to different fusion groups,and they had discrepant pathogenicities to different hosts,but all of them could cause diseases.
分 类 号:S436.43[农业科学—农业昆虫与害虫防治]
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