Map-based cloning of a novel QTL qBN-1 influencing branch number in soybean[Glycine max(L.)Merr.]  

作　　者：Sobhi F.Lamlom Yong Zhang Bohong Su Haitao Wu Xia Zhang Jindong Fu Bo Zhang Li-Juan Qiu 

机构地区：[1]National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization,Ministry of Agriculture,Institute of Crop Sciences,Chinese Academy of Agricultural Science,Beijing 100081,China [2]Plant Production Department,Faculty of Agriculture Saba Basha,Alexandria University,Alexandria 21531,Egypt [3]Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Keshan 161606,Heilongjiang,China [4]College of Agronomy,Northeast Agricultural University,Harbin 150030,Heilongjiang,China [5]School of Plant and Environmental Sciences,Virginia Polytechnic Institute and State University,Blacksburg,VA 24060,USA

出　　处：《The Crop Journal》2020年第5期793-801,共9页作物学报（英文版）

基　　金：This research was supported by the National Key Research and Development Program of China(2016YFD0100201 and 2016YFD0100304);the Platform of National Crop Germplasm Resources of China(2016-004 and 2017-004);the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences.

摘　　要：Branch number(BN)is an important agronomic attribute related to the plant architecture,adaptability,and yield of soybean.To date,few studies ofBNhave been conducted to elucidate its genetic background.We aimed to localize genetic factors affecting BN using segregating populations derived fromthe high-branching cultivar‘Kennong24’(KN24)and the low-branching cultivar‘Kenfeng19’(KF19).Composite interval mapping analysis detected a QTL(qBN-1)on chromosome 6 between the SSR markers BARCSOYSSR_06_0993 and BARCSOYSSR_06_1070 using an F2 population.To fine-map qBN-1,a RIL population was developed and genotyped with 14 SSRmarkers located in the QTL region.qBN-1 was localized to a 115.67-kb interval flanked by markers BARCSOYSSR_06_1048 and BARCSOYSSR_06_1053.The QTL was further confirmed using backcross populations of size 1305(BC2F2 with KN24 as a recurrent parent)and 1712(BC3F2 with KF19 as a recurrent parent).The fine-mapping region of qBN-1 contained only two candidate genes,Glyma.06G208800 and Glyma.06G208900,whose expression patterns were investigated by qRT-PCR.Compared to Glyma.06G208800 gene expression,Glyma.06G208900 showed the highest expression of the two genes and showed a significant difference in expression between high-and low-branching genotypes in either axillary meristem or shoot apical meristem,and showed opposite expression patterns in the two tissues at V4 and R1 stages.These results identify Glyma.06G208900 as a novel candidate gene controlling BN.Taken together,the results of this study provide a foundation for cloning and functional analysis of the qBN-1 gene and for the improvement of BN bymarker-assisted selection in soybean breeding.

关 键 词：SOYBEAN CULTIVAR GLYCINE 

分 类 号：S565.1[农业科学—作物学]