综合放血法所致血虚证小鼠模型的比较研究  

Comparative study of mouse model of blood deficiency pattern caused by comprehensive bloodletting

作　　者：胡柳 韩铨 吴妍 王威 李栋 HU Liu;HAN Quan;WU Yan;WANG Wei;LI Dong(Zhejiang Yangshengtang Natural Medicine Research Institute Co.Ltd.,Zhejiang Province,Hangzhou310024,China)

机构地区：[1]浙江养生堂天然药物研究所有限公司,浙江杭州310024

出　　处：《中国当代医药》2020年第26期35-38,共4页China Modern Medicine

摘　　要：目的通过比较不同造模参数的综合放血方法,确定血虚模型的最优造模参数,为该方法的可重复标准化提供数据支持。方法将100只ICR小鼠随机分为5组:正常对照组(n=20)、模型组A(每日游泳15 min+隔日放血0.3 ml,n=20)、模型组B(每日游泳10 min+隔日放血0.3 ml,n=20),模型组C(每日游泳15 min+隔日放血0.5 ml,n=20)以及模型组D(每日游泳10 min+隔日放血0.5 ml,n=20),造模9 d后取胸腺、脾脏以及血液,测定5组小鼠的体重、免疫器官指数、死亡率、血常规指标、血清免疫因子白细胞介素-6(IL-6)水平及红细胞膜Na+-K+-ATP酶活性。结果模型组A、B、C、D小鼠造模9 d后的体重、免疫器官指数、血常规指标(红细胞、白细胞、血红蛋白和血小板)、血清IL-6水平和红细胞膜Na+-K+-ATP酶活性均低于正常对照组,差异有统计学意义(P<0.05);模型组B小鼠造模9 d后的死亡率低于模型组A、C和D,差异有统计学意义(P<0.05)。结论每日游泳10 min+隔日放血0.3 ml为较优的中医血虚证候模型的造模参数。Objective To compare the comprehensive bloodletting in different modeling parameters to determine the optimal one with blood deficiency pattern,and to provide data support for the repeatable standardization of the method.Methods A total of 100 ICR mice were randomly divided into 5 groups:normal control group(n=20),model group A(15 min swimming daily+bloodletting 0.3 ml every other day,n=20),model group B(10 min swimming daily+bloodletting 0.3 ml every other day,n=20),model group C(15 min swimming daily+bloodletting 0.5 ml every other day,n=20)and model group D(10 min swimming daily+bloodletting 0.5 ml every other day,n=20).Thymus,spleen and blood were collected for measuring the weight,immune organ index,blood routine indicators(including red blood cells,leukocytes,hemoglobin and platelets),serum interleukin-6(IL-6)levels and erythrocyte membrane Na+-K+-ATPase activity after nine days model establishment.Results The weight,immune organ index,blood routine indicators(including red blood cells,leukocytes,hemoglobin and platelets),serum IL-6 levels and erythrocyte membrane Na+-K+-ATPase activity in the model group A,B,C,and D were lower than those in the normal control group after nine days model establishment,and the differences were statistically significant(P<0.05).The animal mortality rate of the model group B was lower than that of model group A,C,and D,and the differences were statistically significant(P<0.05).Conclusion Daily swimming for 10 min+bloodletting 0.3 ml every other day is the optimal model parameters for traditional Chinese medicine syndrome of blood deficiency pattern.

关 键 词：综合放血法 血虚证 中医证候模型 

分 类 号：R332[医药卫生—人体生理学]