健康人口服盐酸黄连素片剂后的尿药分析与药物代谢初步研究  

Determination and Preliminary Studies of Metabolism of Berberine in Human Urine After Oral Administration

作　　者：余琛[1] 张慧[1] 洪有采[1] 任建英[1] 潘俊芳[2] 朱大元 徐修容 

机构地区：[1]上海市徐汇区中心医院,上海200031 [2]中国科学院上海药物研究所,上海200031

出　　处：《中国临床药理学杂志》2000年第1期36-39,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：本文以１３一甲基小檗碱为内标，用正相高效液相色谱一荧光检测的方法定量分析尿液中的小檗碱。考察了健康人一次口服０．２ｇ盐酸黄连素糖衣片后９６ｈ内小檗碱尿药排泄量变化，按矩量法求得其体内药物平均滞留时间（ＭＲＴ）为３２．６３ ±６． １５ ｈ，累积尿药排泄量为 ２７．４９ ± ４．７４ ｕ ｇ；首次检测到小檗碱在人体内有四个未知的代谢峰。A normal phase liquid chromatographic method (flurorescent detector) for determinating berberine (BER) concentration in human urine was reported. The 13-methylberberine was used as the internal standard. A mixture of dichloromethane - methanoldiethyl amine-acetic acid (90: 9:0:4:0.5) was served as the mobile phase. The dynamic change of excretion of BER in urine after oral administration of 0.2 g BER.HCL tablets in single dosage was observed and MRT and AUC was calculed to be 32.63 ± 6. 15 h and 27.49 ± 4.74 u g by statistical moment theory. Furthermore there were four metabolites of berberine chloride in human urine were detected for the first time.

关 键 词：盐酸黄连素片 小檗碱 高效液相色谱法 药物代谢 血药浓度 

分 类 号：R285.6[医药卫生—中药学]