Characterization of inthomycin biosynthetic gene cluster revealing new insights into carboxamide formation  

作　　者：WANG Yong-Jiang WANG Li HE Xin XU Dong-Dong TANG Jun MA Ya-Tuan YAN Yi-Jun GU Yu-Cheng YANG Jing HUANG Sheng-Xiong 

机构地区：[1]State Key Laboratory of Phytochemistry and P/ant Resources in West China,and CAS Center for Excellence in Molecular Plant Sciences,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201.China [2]College of Chemistry and Pharmacy,Norhwest Agriculture and Forestry University,Yangling 712100.China [3]Savaid Medical School,University of Chinese Academy of Scienrces,Bejing 100049.China [4]Syngenta Jelott's Hill International Research Centre,Bracknell,Berkshire RG426EY,U.K.

出　　处：《Chinese Journal of Natural Medicines》2020年第9期677-683,共7页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. U1702285,21708045 and21502203);the Natural Science Foundation of Yunnan Province(Nos. 2019FJ007,2019FA034 and 2018HC012);the Key Research Program of Frontier Sciences and the Strategic Priority Research Program,CAS (Nos. QYZDB-SSW-SMC051 and XDB27020205);the Syngenta Postgraduate Studentship Awarded to WANG Yong-Jiang (2019-2020)。

摘　　要：Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B(1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the ^(15)N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.

关 键 词：Inthomycins BIOSYNTHESIS Gene cluster CARBOXAMIDE P450 

分 类 号：R284[医药卫生—中药学]