贮存式自体输血对家兔骨髓造血干细胞功能和外周血网织红细胞恢复情况的影响  

Effect of stored autologous blood transfusion on function of bone marrow hematopoietic stem cells and recovery of reticulocytes in peripheral blood of rabbits

作　　者：周循 王欢 段立双 李振洲 郭建荣 Zhou Xun;Wang Huan;Duan Lishuang;Li Zhenzhou;Guo Jianrong(Department of Anesthesiology,Gongli Hospital,Naval Military Medical University,Shanghai 200135,China)

机构地区：[1]海军军医大学附属公利医院麻醉科,上海200135

出　　处：《中华麻醉学杂志》2020年第6期732-736,共5页Chinese Journal of Anesthesiology

基　　金：上海市浦东新区卫生系统重点学科群建设项目(PWZxq2017-10);国家自然科学基金面上项目(81671919,81870147)。

摘　　要：目的:评价贮存式自体输血对家兔骨髓造血干细胞功能及外周血网织红细胞恢复情况的影响。方法:健康雄性新西兰家兔30只,3~6月龄,体重2.5~3.0 kg,采用随机数字表法分为5组(n=6):对照组(C组)、手术组(O组)、手术+采血组(O+B组)、手术+贮存式自体全血组(O+WB组)和手术+贮存式自体成分血组(O+CB组)。O+B组、O+WB组和O+CB组于术前7 d时以3~5 ml/min的速率经右股动脉采血,采血量为总血容量的10%,4~6℃贮存;O+CB组血液离心去除血浆和白细胞,制备红细胞悬液,置于贮血袋中。O组、O+B组、O+WB组和O+CB组行肝切除术,经右股动脉以3~5 ml/min的速率放血,放血量为总血容量的10%。O组和O+B组术中静脉输注乳酸林格液1 ml/min,O+WB组术中输注贮存式自体全血,O+CB组术中输注贮存式自体红细胞悬液1 ml/min。分别于手术开始前即刻(T 2)、术后6 h(T 3)和术后24 h(T 4)时采集骨髓血标本,测定CD34^+细胞百分比、细胞周期和端粒DNA相对表达量;分别于自体血贮存前(T 1)和T 4时采集外周静脉血标本,测定网织红细胞百分比。结果:与C组比较,O组T 3,4时、O+B组、O+WB组和O+CB组T 2-4时CD34^+细胞百分比升高,G 1期CD34^+细胞百分比降低,CD34^+细胞端粒DNA表达上调,其余4组T 4时外周血网织红细胞百分比升高(P<0.05);与O组比较,O+B组、O+WB组和O+CB组T 2-4时CD34^+细胞百分比升高,G 1期CD34^+细胞百分比降低,CD34^+细胞端粒DNA表达上调,O+WB组和O+CB组T 4时外周血网织红细胞百分比降低(P<0.05);与O+B组比较,O+WB组和O+CB组T 3,4时CD34^+细胞百分比升高,G 1期CD34^+细胞百分比降低,CD34^+细胞端粒DNA表达上调,O+WB组和O+CB组T 4时外周血网织红细胞百分比降低(P<0.05);与O+WB组比较,O+CB组T 3,4时CD34^+细胞百分比降低,G 1期CD34^+细胞百分比升高,CD34^+细胞端粒DNA表达下调,T 4时外周血网织红细胞百分比升高(P<0.05)。结论:贮存式自体输血可抑制家兔骨髓造血干细胞�Objective To evaluate the effect of stored autologous blood transfusion on the function of bone marrow hematopoietic stem cells and the recovery of reticulocytes in peripheral blood of rabbits.Methods Thirty healthy male New Zealand rabbits,aged 3-6 months,weighing 2.5-3.0 kg,were divided into 5 groups(n=6 each)using a random number table method:control group(C group),operation group(O group),blood collection group(O+B group),stored autologous whole blood group(O+WB group)and stored autologous component blood group(O+CB group).In O+B group,O+WB group and O+CB group,blood was collected via the right femoral artery at a rate of 3-5 ml/min at 7 days before surgery,the blood volume was 10%of the total blood volume,and blood was stored at 4-6℃.In O+CB group,blood was centrifuged to remove plasma and white blood cells,and red blood cell suspension was prepared and placed in a blood storage bag.In O,O+B,O+WB and O+CB groups,hepatectomy was performed,and bleeding was performed through the right femoral artery at a rate of 3-5 ml/min,and the blood volume was 10%of the total blood volume.Lactated Ringer′s solution 1 ml/min was intravenously infused during surgery in O and O+B groups,stored autologous whole blood was intravenously infused during surgery in group O+WB,and stored autologous red blood cell suspension 1 ml/min was intravenously infused during surgery in group O+CB.Bone marrow blood samples were collected immediately before the start of surgery(T2)and at 6 and 24 h after surgery(T3,4)to determine the percentage of CD34^+cells,cell cycle and relative expression of telomere DNA.Peripheral venous blood samples were collected before storage(T1)and at T4 for determination of the percentage of reticulocytes.Results Compared with group C,the percentage of CD34^+cells was significantly increased at T3 and T4,the percentage of CD34^+cells in G1 phase was decreased,and the expression of telomere DNA in CD34^+cells was up-regulated in group O,and the percentage of reticulocyte in peripheral blood was increased at T4 in

关 键 词：输血 自体 造血干细胞 网状细胞 

分 类 号：R457.1[医药卫生—治疗学]