丙泊酚对小鼠眶额叶皮层锥体神经元兴奋性的影响及其离子通道机制  

Effect of propofol on excitability of pyramidal neurons in orbitofrontal cortex of mice and underlying ion channel mechanism

作　　者：丁伟星 陈杨 张铭 易斌 鲁开智 王进全 Ding Weixing;Chen Yang;Zhang Ming;Yi Bin;Lu Kaizhi;Wang Jinquan(Department of Anesthesiology,Qujing Second People's Hospital of Yunnan Province,Qujing 655000,China;Department of Anesthesiology,First Affiliated Hospital,Military Medical University of the Army,Chongqing 400038,China)

机构地区：[1]云南省曲靖市第二人民医院麻醉科,655000 [2]陆军军医大学第一附属医院手术麻醉科,重庆400038

出　　处：《中华麻醉学杂志》2020年第5期577-580,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81600035)。

摘　　要：目的评价丙泊酚对小鼠眶额叶皮层锥体神经元兴奋性的影响及其离子通道机制。方法健康雄性C57小鼠,8~12周龄,制备400μm厚的脑片。实验Ⅰ采用随机数字表法将脑片分为2组(n=7):对照组(C组)细胞外灌流丙泊酚溶剂2 min;丙泊酚组(P组)细胞外灌流10μmol/L丙泊酚。实验Ⅱ采用随机数字表法将脑片分为5组(n=8):丙泊酚组(P组)细胞外灌流10μmol/L丙泊酚2 min;超极化激活非选择性阳离子通道阻断剂ZD7288+丙泊酚组(Z+P组)、内向整流钾通道阻断剂托肽品+丙泊酚组(T+P组)、瞬时激活电压门控钾通道阻断剂4AP+丙泊酚组(A+P组)和延迟激活电压门控钾通道阻断剂TEA+丙泊酚组(TEA+P组)细胞外分别灌流5μmol/L ZD7288和10μmol/L丙泊酚2 min、5μmol/L托肽品和10μmol/L丙泊酚2 min、10μmol/L 4AP和10μmol/L丙泊酚2 min、10μmol/L TEA和10μmol/L丙泊酚2 min。采用全细胞膜片钳法检测眶额叶锥体神经元全细胞电流、膜电位和放电频率。结果实验Ⅰ与C组比较,P组全细胞电流升高,膜电位和放电频率降低(P<0.01)。实验Ⅱ与P组比较,Z+P组、T+P组和A+P组全细胞电流、膜电位和放电频率差异无统计学意义(P>0.05),TEA+P组全细胞电流和膜电位降低,放电频率升高(P<0.01)。结论丙泊酚可抑制小鼠眶额叶皮层锥体神经元兴奋性,其机制与激活延迟激活电压门控钾通道有关。Objective To evaluate the effect of propofol on excitability of pyramidal neurons in orbitofrontal cortex of mice and the underlying ion channel mechanism.Methods Brain slices of 400μm thickness from healthy male C57 mice(aged 8-12 weeks)were prepared.This experiment was performed in two parts.PartⅠThe brain slices were divided into 2 groups(n=7 each)based on the random number table method:control group(C group)and propofol group(P group).Cells were perfused with vehicle in group C and with 10μmol/L propofol in group P.PartⅡThe brain slices were divided into 5 groups(n=8 each)using the random number table method:propofol group(P group),hyperpolarization-activated non-selective cation channel antagonist ZD7288 plus propofol group(Z+P group),inward rectifier potassium channel antagonist topiramate plus propofol group(T+P group),transient activation of voltage-gated potassium channel antagonist 4-aminopyridine(4AP)plus propofol group(A+P group),and delayed activation of voltage-gated potassium channel antagonist tetraethylammonium(TEA)plus propofol group(TEA+P group).Cells were perfused with 10μmol/L propofol for 2 min in P group,with 5μmol/L ZD7288 and 10μmol/L melatonin for 2 min in Z+P group,with 5μmol/L topiramate and 10μmol/L propofol for 2 min in T+P group,with 10μmol/L 4-aminopyridine and 10μmol/L propofol for 2 min in A+P group,and with 10μmol/L TEA and 10μmol/L propofol for 2 min in TEA+P group.The whole-cell currents,membrane potential and discharge frequency of pyramidal neurons in the orbitofrontal cortex were recorded by whole-cell patch-clamp.Results PartⅠCompared with C group,whole-cell currents were significantly increased,and the membrane potential and discharge frequency were decreased in P group(P<0.01).PartⅡCompared with P group,no significant change was found in the whole-cell currents,membrane potentials and discharge frequency in Z+P group,T+P group and A+P group(P>0.05),and the whole-cell currents were significantly decreased,and the membrane potentials and discharge freque

关 键 词：二异丙酚 额叶 神经元 

分 类 号：R614[医药卫生—麻醉学]