胶原海绵与脱细胞基质在大鼠体内的降解过程  

Degradation process of collagen sponge and acellular matrix implanted in rat

作　　者：马也 赵磊磊 高建萍[2] 孔英俊[2] 张贵锋[2] 刘涛 MA Ye;ZHAO Lei-lei;GAO Jian-ping;KONG Ying-jun;ZHANG Gui-feng;LIU Tao(College of Life Science and Biopharmaceuticals,Guangdong Pharmaceutical University,Guangzhou 510000;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,CAS,Beijing 100190;Hebei Collison Biotechnology Co.,Ltd.,Handan 057450,China)

机构地区：[1]广东药科大学生命科学与生物制药学院,广州510000 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]河北考力森生物科技有限公司,邯郸057450

出　　处：《生物学杂志》2020年第5期112-116,共5页Journal of Biology

基　　金：国家重点研发计划(2018YFA0108200,2018YFC1106400);广州市科技计划项目(201803010086);海口市海洋经济创新发展示范城市产业链协调创新类项目(HHCL201804)。

摘　　要：针对胶原基生物材料,通过不同方法研究了基于牛胶原的海绵和脱细胞基质材料的降解过程,比较两种材料的降解规律,定量考察了胶原基材料体内降解周期。通过在SD大鼠脊柱两侧皮下分别植入胶原海绵与脱细胞基质,在不同周数取样,分别观察两种材料降解程度并称重建立降解曲线,利用高效液相色谱质谱联用技术筛选出牛I型胶原的特征多肽GEGGPQGPRG,并进行定量分析。分批次将大鼠体内残留部分进行酶解,再利用三重四极杆质谱对特征肽进行定量,建立降解曲线。结果表明,脱细胞基质与胶原海绵体内降解趋势相似。胶原海绵降解8周特征肽浓度为(0.40±0.10)×10^-5 mg/mL,表明胶原海绵降解较为完全。脱细胞基质降解16周特征肽浓度为(1.30±0.11)×10^-5 mg/mL,表明脱细胞基质降解较为完全。For collagen-based biomaterials,the degradation processes of bovine collagen-based sponges and acellular matrix(ACM)materials were studied by different methods.The degradation laws of the two materials were compared,and the degradation cycle of collagen-based materials in vivo was quantitatively examined.Collagen sponges and ACM were implanted subcutaneously on both sides of the spine of SD rats,and samples were taken at different weeks to observe the degree of degradation of the two materials and weighed to establish a degradation curve.The characteristic peptide GEGGPQGPRG of bovine type I collagen was quantified.The remaining part of the rat body was digested in batches,and the characteristic peptide was quantified by triple quadrupole mass spectrometry to establish a degradation curve.The results showed that the ACM matrix and collagen sponge had the same degradation trend.The collagen sponge was degraded for 8 weeks,and the characteristic peptide concentration was(0.40±0.10)×10^-5 mg/mL,indicating that the collagen sponge was completely degraded.The acellular matrix was degraded for 16 weeks,and the characteristic peptide concentration was(1.30±0.11)×10^-5 mg/mL,indicating that the acellular matrix was completely degraded.

关 键 词：脱细胞基质 胶原海绵 体内降解 液质联用技术 特征多肽 

分 类 号：R318.08[医药卫生—生物医学工程]