基于体外抑菌活性的艾粉黄酮富集工艺研究  

Study on the enrichment technology of the flavonoids from Artemisia Argyi based on the antibacterial activity in vitro

作　　者：贾雅婷 许舒娅 李利红[1] 吴召运 JIA Yating;XU Shuya;LI Lihong;WU Zhaoyun(College of Animal medicine,Henan University of Animal Husbandary and Economy,Zhengzhou Henan 450046;Nan Yang Bencaoai Development Co.,Ltd,Fangcheng Henan 473200)

机构地区：[1]河南牧业经济学院动物医药学院,河南郑州450046 [2]南阳市本草艾开发有限公司,河南方城473200

出　　处：《现代牧业》2020年第3期6-9,共4页Modern Animal Husbandry

基　　金：河南牧业经济学院重点培育学科资助项目(41000003)。

摘　　要：为充分开发利用艾叶粉,本研究以打孔法进行艾粉提取物抑菌活性测定。采用微量倍比稀释法进行最小抑菌浓度(MIC)测定;以硅藻土柱层析法进行黄酮的富集;采用紫外分光光度法,以芦丁为标准品,测定黄酮的含量。结果显示艾粉70%乙醇提取物对金黄色葡萄球菌的抑菌效果最好,抑菌圈直径达18.6mm,MIC为31.25mg/mL。硅藻土柱层析法富集艾粉70%乙醇提取物中的黄酮,含量从19.92%上升至27.86%。In this study,the antibacterial activity of Artemisia Argyi extract was determined by drilling method,the minimum inhibitory concentration(MIC)was determined by micro multiple dilution method,the flavonoids were enriched by diatomite column chromatography,and the contents of flavonoids were determined by UV spectrophotometry with Rutin as reference substance.The results showed that the 70%ethanol extract of Artemisia Argyi powder had the best bacteriostatic effect on Staphylococcus aureus.The diameter of inhibition circle was 18.6 mm and MIC was 31.25 mg/ml.The content of flavonoids increased from 19.92%to 27.86%by diatomite column chromatography.This study provides a reference for the development and utilization of Artemisia argyi powder.

关 键 词：艾粉 金黄色葡萄球菌 黄酮 富集 

分 类 号：Q935[生物学—微生物学]