基于IRS-1信号通路探讨虎杖甙对2型糖尿病大鼠胰岛素抵抗的改善作用  

Polydatin Has Effect on Improving Insulin Resistance in Rats with Type 2 Diabetes Based on IRS-1 Signaling Pathway

作　　者：应巧[1] 何斐[1] 张伟[1] 泮瑛瑛 张霞霞 YING Qiao;HE Fei;ZHANG Wei;PAN Yingying;ZHANG Xiaxia

机构地区：[1]台州市立医院内分泌科,浙江台州318000

出　　处：《新中医》2020年第17期4-8,共5页New Chinese Medicine

基　　金：台州市科技计划项目(1902ky50)。

摘　　要：目的:探讨虎杖甙对2型糖尿病大鼠胰岛素抵抗(IR)的改善作用及其作用机制。方法:SPF级SD大鼠100只,随机分为对照组,模型组,吡咯列酮组,虎杖甙低、高剂量组5组,每组20只。除对照组外,其余各组大鼠腹腔注射链脲佐菌素建立2型糖尿病大鼠模型。吡咯列酮组,虎杖甙低、高剂量组于模型制备成功后第1天开始灌胃相应药物,对照组和模型组灌胃等体积生理盐水。试验结束后,测定IR相关指标,RT-PCR法及Western blot法测定胰腺胰岛素受体底物-1(IRS-1)、葡萄糖转运蛋白-4(GLUT-4)基因和蛋白水平。结果:与对照组比较,模型组空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)水平升高(P<0.05),胰岛β细胞功能指数(HOMA-β)、胰岛素敏感性检测指数(QUICKI)水平、胰腺IRS-1、GLUT-4 mRNA及蛋白水平降低(P<0.05)。与模型组比较,吡咯列酮组、虎杖甙低、高剂量组FBG、FINS、HOMA-IR水平降低(P<0.05),HOMA-β、QUICKI水平、胰腺IRS-1、GLUT-4 mRNA及蛋白水平升高(P<0.05)。与吡咯列酮组比较,虎杖甙低、高剂量组FBG、FINS、HOMA-IR水平升高(P<0.05),HOMA-β、QUICKI水平、胰腺IRS-1、GLUT-4 m RNA及蛋白水平降低(P<0.05)。与虎杖甙低剂量组比较,虎杖甙高量组FBG、FINS、HOMA-IR水平降低(P<0.05),HOMA-β、QUICKI水平、胰腺IRS-1、GLUT-4 mRNA及蛋白水平升高(P<0.05)。结论:虎杖甙能明显改善2型糖尿病大鼠IR作用,其机制可能与虎杖甙能促进2型糖尿病大鼠胰腺IRS-1、GLUT-4 m RNA及蛋白表达,进而激活IRS-1信号通路有关。Objective:To discuss improvement effect of polydatin on insulin resistance(IR) in rats with type 2 diabetes and its mechanism. Methods: A total of 100 SD rates at SPF level were randomly divided into the control group, the model group,the pioglitazone group,and the polydatin groups of low and high dose,20 rats in each group. Except the control group, the rat models with type 2 diabetes were established through intraperitoneal injection of streptozotocin in other groups. On the 1 st day after successful establishment,the pioglitazone group and the polydatin groups of low and high dose were given gastric perfusion with corresponding medicine;the control group and the model group were given normal saline of same volume. After experiment,the relevant indexes of IR were determined;the levels of m RNA and protein of insulin receptor substrate-1(IRS-1) in the pancreas and glucose transporter-4(GLUT-4) were determined by RT-PCR method and Western blot method. Results: Compared with those in the control group, the levels of fasting plasma glucose(FBG),fasting insulin(FINS) and homeostasis model assessment of insulin resistance(HOMA-IR) in the model group were increased(P<0.05);the levels of homeostatic model assessment of islet β cell function(HOMA-β) and quantitative insulin sensitivity check index(QUICKI),and the levels of mRNA and protein of IRS-1 in the pancreas and GLUT-4 were decreased(P<0.05).Compared with those in the model group,the levels of FBG,FINS and HOMA-IR in the pioglitazone group and the polydatin groups of low and high dose were decreased(P<0.05);the levels of HOMA-β and QUICKI,and the levels of mRNA and protein of IRS-1 in the pancreas and GLUT-4 were increased(P<0.05). Compared with those in the pioglitazone group,the levels of FBG, FINS and HOMA-IR in the polydatin groups of low and high dose were increased(P<0.05);the levels of HOMA-β and QUICKI,and the levels of mRNA and protein of IRS-1 in the pancreas and GLUT-4 were decreased(P<0.05).Compared with those in the low-dose polydatin group,the le

关 键 词：2型糖尿病 虎杖甙 胰岛素受体底物-1 胰岛素抵抗 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]