白芍提取物调控miR-564表达对白血病细胞增殖及凋亡影响的研究  

Study on Effect of Extract of Radix Paeoniae Alba Regulating miR-564 Expression on Proliferation and Apoptosis of Leukemia Cells

作　　者：王大鹏[1] 杨建[2] 刘现辉[1] WANG Dapeng;YANG Jian;LIU Xianhui

机构地区：[1]河南中医药大学第一附属医院血液肿瘤科,河南郑州450000 [2]河南中医药大学第一附属医院普外科,河南郑州450000

出　　处：《新中医》2020年第17期9-12,共4页New Chinese Medicine

摘　　要：目的:探讨白芍提取物对白血病细胞增殖、凋亡的影响及其作用机制。方法:选用白血病细胞K652为研究对象,分为对照组、白芍提取物不同浓度(20μg/mL、30μg/mL、40μg/mL)组、miR-NC组、miR-564组、白芍提取物30μg/mL+anti-miR-NC组及白芍提取物30μg/mL+anti-miR-564组。采用MTT法检测各组细胞活性,流式细胞术检测各组细胞凋亡情况,实时荧光定量PCR检测细胞中miR-564表达,蛋白质印迹法检测各组细胞中细胞周期蛋白D1(CyclinD1)、细胞周期依赖性蛋白激酶抑制因子1A(P21)、B淋巴细胞瘤-2(Bcl-2)及B淋巴细胞瘤-2相关X蛋白(Bax)的表达。结果:与对照组比较,白芍提取物不同浓度组K652细胞活性、Cyclin D1均降低(P<0.05),P21表达水平升高(P<0.05);白芍提取物30μg/mL组Bcl-2蛋白表达水平降低(P<0.05),细胞凋亡率、Bax蛋白及miR-564表达水平升高(P<0.05)。与miR-NC组比较,miR-564组K652细胞活性、Cyclin D1及Bcl-2蛋白表达水平均降低(P<0.05),细胞凋亡率、P21、Bax蛋白及miR-564表达水平均升高(P<0.05)。与白芍提取物30μg/mL+anti-miR-NC组比较,白芍提取物30μg/mL+anti-miR-564组K652细胞活性、Cyclin D1及Bcl-2蛋白表达水平均升高(P<0.05),细胞凋亡率、P21、Bax蛋白及miR-564表达水平均降低(P<0.05)。白芍提取物30μg/mL+anti-miR-NC组各检测指标与白芍提取物组30μg/mL组比较,差异无统计学意义(P>0.05)。结论:白芍提取物可能通过上调miR-564表达抑制白血病细胞增殖,并诱导细胞凋亡。Objective:To discuss the effect of extract of Radix Paeoniae Alba on proliferation and apoptosis of leukemia cells and its mechanism. Methods: Leukemia cells K652 were selected as the study objects, and were divided into the control group,the extract of Radix Paeoniae Alba groups of different concentrations,namely,20 μg/mL,30 μg/mL and40 μg/mL,the miR-NC group,the miR-564 group,the group of 30 μg/mL extract of Radix Paeoniae Alba combined with anti-miR-NC, and the group of 30 μg/mL extract of Radix Paeoniae Alba combined with anti-miR-564. In each group,activity was detected by MTT method;apoptosis was detected by flow cytometry;the real-time fluorescence quantitative PCR was applied to detect miR-564 expression. In each group, the expression of cyclinD1, cyclin-dependent kinase inhibitor 1 A(P21), B cell lymphoma-2(Bcl-2), and Bcl-2 associated X protein(Bax) were detected by Western blotting.Results:Compared with those in the control group,K652 cell activity,and the expression levels of CyclinD1 in the extract of Radix Paeoniae Alba groups of different concentrations were decreased(P<0.05);the expression levels of P21 was increased(P<0.05);Bcl-2 protein in the 30 μg/mL extract of Radix Paeoniae Alba group were decreased(P<0.05),and the apoptosis rate,Bax protein and miR-564 were increased(P<0.05). Compared with those in the miR-NC group,K652 cell activity,and the expression levels of CyclinD1 and Bcl-2 protein in the miR-564 group were decreased(P<0.05);the apoptosis rate,and the expression levels of P21,Bax protein and miR-564 were increased(P<0.05). Compared with those in the group of 30 μg/mL extract of Radix Paeoniae Alba combined with anti-miR-NC, K652 cell activity, and the expression levels of CyclinD1 and Bcl-2 protein in the group of 30 μg/mL extract of Radix Paeoniae Alba combined with anti-miR-564 were increased(P<0.05);the apoptosis rate,and the expression levels of P21,Bax protein and miR-564 were decreased(P<0.05). There was no significant difference being found in the comparison of each de

关 键 词：白血病 白芍提取物 miR-564 增殖 凋亡 细胞实验 

分 类 号：R285.5[医药卫生—中药学] R733.7[医药卫生—中医学]