大鼠尿道狭窄模型的超声评价方法学探讨  

High-frequency ultrasonography for evaluation of the urethral stricture model in rats

作　　者：叶小剑[1] 许宁[2] YE Xiao-jian;XU Ning(Department of Ultrasonographic Imaging,The First Affiliated Hospital of Fujian Medical University,Fuzhou,Fujian 350005,China;Department of Urology,The First Affiliated Hospital of Fujian Medical University,Fuzhou,Fujian 350005,China)

机构地区：[1]福建医科大学附属第一医院超声影像科,福建福州350005 [2]福建医科大学附属第一医院泌尿外科,福建福州350005

出　　处：《中华男科学杂志》2020年第7期595-600,共6页National Journal of Andrology

摘　　要：目的:探讨大鼠尿道狭窄模型建立的方法学,模型建立终点的影像评价方法及有效性。方法:24只雄性成年大鼠,随机分为空白对照组及手术组,手术组以相同方式切割尿道阴茎段,致使尿道不全断裂,后随机分为尿道海绵体内注射重组转化生长因子-β1(TGF-β1,TGF组)及尿道海绵体内注射TGF-β1及间充质干细胞组(MSC组),术后4周以高频超声结合水浴法盲法评价24只大鼠尿道情况,与实际分组情况做对照。结果:组织病理结果显示,手术组大鼠均出现比空白对照组更明显的尿道狭窄,TGF组尿道纤维组织增生程度较MSC组更严重。超声检查发现,所有手术组大鼠均出现了回声条带变窄(P<0.01),强度减低,部分条带回声模糊等改变。同时根据上述超声改变予以赋值评分,在盲法情况下依据评分结果对手术组大鼠分组,分组结果与实际分组情况相符合。区分TGF组及MSC组的ROC曲线下面积为0.972,超声赋值评分取7.5分时,敏感性100.0%,特异性83.3%。结论:高频超声结合水浴法可以有效评价大鼠尿道狭窄情况,指导模型建立。Objective:To explore the methodology for the establishment of the urethral stricture model in rats,the methods of end-point imaging evaluation of the model establishment and their efficiency.Methods:Twenty-four adult male rats were randomly divided into a blank control(n=6)and a model group(n=18),and the model was established by cutting the penile segment of the urethra to induce incomplete urethral rupture.Then the model rats were treated by injection of transforming growth factor-β1(the TGF group,n=6)or mesenchymal stem cells(the MSC group,n=12)into the urethral cavernosum.At 4 weeks after modeling,the urethral condition of all the rats was evaluated blindly using high-frequency ultrasound combined with water bath,followed by comparison among different groups.Results:Compared with the blank controls,the model rats showed evident urethral stricture,with more significant urethral fibrous tissue hyperplasia in the TGF than in the MSC group.Ultrasonography revealed significantly narrowed echo strips(P<0.01),decreased echo intensity and blurred echoes in some strips.The grouping of the model rats according to the assigned values to the ultrasonographic changes was consistent with the actual condition.The area under the ROC curve for distinguishing between the TGF and MSC groups was 0.972,with a sensitivity of 100.0%and a specificity of 83.3%when ultrasound assignment score was 7.5.Conclusion:High-frequency ultrasonography combined with water bath is effective for evaluating urethral stricture in rats and may give some guidance in the establishment of the urethral stricture model.

关 键 词：尿道狭窄 大鼠模型 高频超声 

分 类 号：R695[医药卫生—泌尿科学]