2013-2019年北京市昌平区霍乱弧菌病原学和分子流行病学特征分析  

Characteristics of phenotype and molecular type of Vibrio cholerae in Changping district of Beijing, 2013–2019

作　　者：舒高林 李东迅[1] 彭华[1] 王维钧[1] 银安琪 牛桓彩[1] 严寒秋[2] Shu Gaolin;Li Dongxun;Peng Hua;Wang Weijun;Yin Anqi;Niu Huancai;Yan Hanqiu(Changping District Center for Disease Control and Prevention,Beijing 102200,China;Beijing Municipal Center for Disease Prevention and Control,Beijing Research Center for Preventive Medicine,Beijing Key Laboratoiy of Diagnostic and Traceability Technologies for Food Poisoning,Beijing 100013,China)

机构地区：[1]北京市昌平区疾病预防控制中心,北京102200 [2]北京市疾病预防控制中心/北京市预防医学研究中心/食物中毒诊断溯源技术北京市重点实验室,北京100013

出　　处：《疾病监测》2020年第8期735-741,共7页Disease Surveillance

基　　金：国家科技重大专项(No.2018ZX10714002–003–002)。

摘　　要：目的了解2013—2019年北京市昌平区霍乱弧菌表型及其分子特征。方法采用常规培养方法对2013—2019年北京市昌平区疑似霍乱标本进行检测。对分离鉴定的O1群小川型霍乱弧菌,用NotⅠ和SfiⅠ两种内切酶进行脉冲场凝胶电泳(PFGE)、用荧光PCR和普通PCR进行9种毒力基因检测,用微量肉汤稀释法进行21种药物敏感试验。结果 2013—2019年北京市昌平区共分离到29株O1群小川型霍乱弧菌,其中散发病例中分离到10株,一起聚集性疫情中分离到19株。29株菌经NotⅠ和SfiⅠ双酶切后可分成3种PFGE谱型(CP-01~03),3种PFGE谱型间的相似系数为78.50%~100.00%,以CP-02为主(27/29,93.10%)。所有菌株均不携带ctxAB基因,携带toxR和hlyA基因,其他毒力基因ompU、tcpA、tcpI、rtxC、zot和ace的携带率分别为89.66%、82.76%、82.76%、44.83%、6.90%和6.90%。29株菌药敏结果,除头孢唑啉(0.00%)和链霉素(3.45%)敏感率极低外,对其他19种抗菌素为敏感(82.76%~100.00%)。结论 2013—2019年北京市昌平区的O1群小川型霍乱弧菌为非产毒株,以PFGE谱型CP-02为主,毒力基因toxR和hlyA携带率高,对多数抗生素敏感,提示在防控工作中应关注此类菌株并制定相应的方针政策,减少疫情的发生。Objectives To understand the characteristics of the phenotype and molecular type of Vibrio cholerae strains in Changping district of Beijing from 2013 to 2019. Methods The conventional test was used for the detection of V. cholerae in suspected samples collected in Changping during this period. All V. cholerae O1 serotype Ogawa strains isolated were digested by two restrict enzyme(Not Ⅰ and SfiⅠ), and then analyzed with pulsed field gel electrophoresis. Nine virulence genes were detected by real-time PCR and PCR. The strains’ susceptibilities to 21 antibiotics were tested by minimal inhibitory concentration. Results A total of 29 V. cholerae O1 serotype Ogawa strains(10 strains isolated from sporadic cases, 19 strains isolated from an outbreak) were isolated in Changping from 2013 to 2019. Among the 29 V. cholerae strains, three PFGE patterns(CP-01–03) were found with the similarity coefficient from 78.5% to 100%, and the CP-02(27/29, 93.10%) was the predominant PFGE pattern. The gene toxR and hlyA were found in all the strains, but no ctxAB was found. The carriage rates of gene ompU, tcpA, tcpI, rtxC, zot and ace were 89.66%, 82.76%, 82.76%, 44.83%, 6.90% and 6.90%, respectively.Except cefazolin(0.00%) and streptomycin(3.45%), most V. cholerae strains were sensitive to the other 19 antibiotics(82.76%–100.00%). Conclusions All the V. cholerae O1 serotype Ogawa strains isolated in Changping from 2013 to 2019 were non-toxigenic. Most V. cholerae O1 serotype Ogawa strains belonged to the same PFGE pattern. The carriage rates of gene toxR and hlyA were higher. The strains showed sensitivity to most antibiotics. Our data suggested that more attention should be paid to such strains and targeted measure are necessary in the future prevention and control to reduce cholera outbreak.

关 键 词：霍乱弧菌 脉冲场凝胶电泳 毒力基因 耐药性 

分 类 号：R211[医药卫生—中医学] R516.5