大豆不同生育期叶际光合细菌群落结构特征  

Community structure characteristics of soybean phyllosphere photosynthetic bacteria in different growth stages

作　　者：孔小婷 苏品 程菊娥[2] 杜晓华[2] 罗路云 陈丽洁 翟忠英 张德咏 刘勇 KONG Xiao-ting;SU Pin;CHENG Ju-e;DU Xiao-hua;LUO Lu-yun;CHEN Li-jie;ZHAI Zhong-ying;ZHANG De-yong;LIU Yong(Longping Branch,Graduate School of Hunan University,Changsha 410125,China;Institute of Plant Protection,Hunan Academy of Agriculture Sciences,Changsha 410125,China)

机构地区：[1]湖南大学隆平分院,长沙410125 [2]湖南省农业科学院植物保护研究所,长沙410125

出　　处：《南方农业学报》2020年第8期1977-1984,共8页Journal of Southern Agriculture

基　　金：国家自然科学基金青年科学基金项目(31701764)。

摘　　要：【目的】探究大豆不同生育期叶际光合细菌群落结构多样性及其分布特征,揭示大豆生育期与光合细菌群落结构变化间的关系,为促进光合细菌在农业生产中的应用提供参考依据。【方法】对5个生育期(苗期、出枝期、花期、鼓粒期和成熟期)大豆叶际光合细菌的Puf M基因序列进行PCR扩增,并对PCR扩增产物进行Illumina高通量测序,分析大豆叶际光合细菌群落多样性及分布特征。【结果】从大豆5个生育期叶片样品中共检测到光合细菌2门、5纲、36属、84种。不同生育期大豆叶片样品中光合细菌的群落组成和结构存在一定差异,在相对丰度方面表现为成熟期>苗期>出枝期>花期>鼓粒期;在多样性方面表现为苗期>出枝期>花期>鼓粒期>成熟期。大豆不同生育期的叶际光合细菌在门、纲、属和种分类水平上的优势菌群及所占比例分别为变形菌门(Proteobacteria)(92.11%~99.29%)、α-变形菌纲(Alphaproteobacteria)(75.17%~97.40%)、甲基杆菌属(Methylobacterium)(60.67%~95.09%)和扭脱甲基杆菌(Methylobacterium extorquens)(36.89%~87.88%),随着分类水平逐渐细化,不同生育期对大豆叶际光合细菌群落组成和分布的影响越大。【结论】生育期对大豆叶际光合细菌群落结构有重要影响,实际生产中可通过在大豆不同生育期施用不同的光合细菌以促进优势群落形成,从而促进大豆生长。【Objective】To explore the phyllosphere photosynthetic bacterial communities structure diversity and distribution of soybean at different growth stages,reveal the relationship between the growth period of soybean and the changes of photosynthetic bacteria community structure,provide a scientific reference for promoting the application of photosynthetic bacteria in agricultural production.【Method】The Puf M gene region of phyllosphere photosynthetic bacteria in five growth stages of soybean(seedling stages,branching stages,flowering stages,bulging stages and maturity stages)was amplified by PCR,and then the products of PCR amplification were sequenced with Illumina high throughput to analyze the diversity and distribution of phyllosphere photosynthetic bacteria.【Result】A total of 84 species,36 genera,5 classes and 2 phyla of photosynthetic bacteria were detected in the leaves samples from five growth stages.There were some differences in the structure and composition of photosynthetic bacterial community in soybean leaves samples of different growth stages.In terms of relative abundance,maturity stages>seedling stages>branching stages>flowering stages>bulging stages;while in terms of diversity,seedling stages>branching stages>flowering stages>bulging stages>maturity stages.The dominant phyllosphere photosynthetic bacterial community at phyla,class,genus and species levels of five growth stages soybean and their proportions were Proteobacteria(92.11%-99.29%),Alphaproteobacteria(75.17%-97.40%),Methylobacterium(60.67%-95.09%),Methylobacterium extorquens(36.89%-87.88%).However,with the refinement of classification,the influence of growth stages on the composition and distribution of phyllosphere photosynthetic bacteria community became great.【Conclusion】Soybean growth periods have an important influence on the structure of phyllosphere photosynthetic bacteria community.In agricultural production,different photosynthetic bacteria could be applied in different growth stages of soybean to promote the format

关 键 词：大豆 生育期 叶际 光合细菌 高通量测序 多样性 

分 类 号：S565.1[农业科学—作物学]