拉曼光谱技术在鉴别瑞氏-姬姆萨染色的肿瘤细胞与正常细胞中的应用  

Application of Raman spectroscopy in the identification of Wright-Giemsa stained tumor cells and normal cells

作　　者：李浩 于华杰 李胜 韩露[2] 沈阳洋 LI Hao;YU Huajie;LI Sheng;HAN Lu;SHEN Yangyang(Shandong Pharmaceutical Research Institute,Jinan 250062,China;Shangdong Cancer Hospital and Institute,Jinan 250117;School of Medicine and Life Sciences,University of Jinan,Jinan 250220;Shandong Academy of Medical Sciences,Jinan 250062)

机构地区：[1]山东省药物研究院,济南250062 [2]山东省肿瘤防治研究院,济南250117 [3]济南大学医学与生命科学学院,济南250220 [4]山东省医学科学院,济南250062

出　　处：《生物医学工程研究》2020年第3期309-313,共5页Journal Of Biomedical Engineering Research

基　　金：山东省重大科技创新工程项目(2017CXGC1204)。

摘　　要：为探究利用显微共聚焦拉曼光谱仪辨别经瑞氏-姬姆萨染色后的肿瘤细胞与正常细胞的可行性。应用拉曼光谱技术在532 nm波长激光下分别检测经瑞氏-姬姆萨染色技术染色的肿瘤细胞与正常细胞,对比6种肿瘤细胞、正常细胞和瑞氏-姬姆萨染料的拉曼光谱特征峰值,正常细胞和肿瘤细胞在208~215 cm^-1、278~285 cm^-1、336~341 cm^-1峰位均有拉曼特征峰。发现6种肿瘤细胞的拉曼光谱峰值和瑞氏-姬姆萨染料的拉曼信号峰值均相同。经分析认为,检测到的均为各种染料的拉曼信号而非6种肿瘤细胞的拉曼信号。因此,无法用显微共聚焦拉曼光谱仪区分经瑞氏-姬姆萨染色后的肿瘤细胞与正常细胞。To explore the feasibility of using micro confocal Raman spectrometer to discriminate tumor cells and normal cells stained by Wright-Giemsa dye,we used Raman spectroscopy to detect the tumor cells and normal cells with Wright-Giemsa stain under 532 nm wavelength laser,and compared the Raman spectral characteristic peaks of 6 kinds of tumor cells,normal cells and Wright-Giemsa dye.We found that the Raman peaks of 6 kinds tumor cells were the same as those of Wright-Giemsa dye.The results showed that the detected Raman signals were the Ramam signals of Wright-Giemsa dye,not those of 6 kinds tumor cells.Therefore,it is impossible to discriminate tumor cells and normal cells by using micro confocal Raman spectrometer.

关 键 词：拉曼光谱技术 拉曼光谱特征峰 瑞氏-姬姆萨染色 肿瘤细胞 正常细胞 

分 类 号：R318[医药卫生—生物医学工程]