小黑杨MYB122基因生物信息学及表达  

Bioinformatics and Expression Analysis of MYB122 Gene in Populus simonii×P.nigra

作　　者：吕冠斌 赵凯[1] 刘悦 姜廷波[1] 周博如[1] Lü Guanbin;Zhao Kai;Liu Yue;Jiang Tingbo;Zhou Boru(State Key Laboratory of Forest Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,P.R.China)

机构地区：[1]林木遗传育种国家重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2020年第9期26-29,40,共5页Journal of Northeast Forestry University

基　　金：转基因生物新品种培育重大专项项目(2018ZX08020002)。

摘　　要：为解析林木抗逆胁迫分子机制、培育抗性转基因林木,以小黑杨(Populus simonii×P.nigra)为试材,同源克隆出954 bp的MYB122(Potri.008G122100.1)基因cDNA,该基因编码317个氨基酸,该蛋白属热稳定性较低的亲水性蛋白。RT-qPCR分析表明,盐胁迫下MYB122基因在根中表达水平明显提高,并且盐胁迫12 h相对表达量达到最高水平。亚细胞定位分析表明,MYB122编码的蛋白质定位在细胞核中。自激活验证结果显示,MYB122蛋白质有自激活活性,且该基因的激活区域在C端1~60个氨基酸之间。A 954 bp MYB122(Potri.008 G122100.1)gene cDNA was cloned from Populus simonii(P.simonii×P.nigra),encoding 317 amino acids,which is a hydrophilic protein with low thermal stability.RT-qPCR analysis showed that the expression level of MYB122 gene in roots increased significantly under salt stress,and the relative expression level reached the highest level in 12 h under salt stress.Subcellular localization analysis showed that the protein encoded by MYB122 was localized in the nucleus.The result of self-activation verification showed that the MYB122 protein has self-activation activity,and the activation region of this gene is between 1-60 amino acid of C-terminal.

关 键 词：小黑杨 MYB转录因子 盐胁迫应答 亚细胞定位 自激活验证 

分 类 号：S792.11[农业科学—林木遗传育种] S722.36[农业科学—林学]