壳低聚糖对PM2.5所致急性肺损伤的保护作用研究  

Protective effect of chitosan oligosaccharides on PM2.5-induced acute lung injury

作　　者：游航 唐华 唐华 YOU Hang;TANG Hua;TANG Hua(School of Clinical Medcine,Southwest Medical University,Luzhou Sichuan 646000,China;Department of Pathophysiology,School of Basic Medical Sciences,Southwest Medical University,Luzhou Sichuan 646000,China)

机构地区：[1]西南医科大学临床医学院,四川泸州646000 [2]西南医科大学病理生理教研室,四川泸州646000

出　　处：《转化医学杂志》2020年第5期262-265,295,共5页Translational Medicine Journal

基　　金：国家级大学生创新创业课题(201710632048)。

摘　　要：目的探讨壳低聚糖对PM2.5暴露后急性肺损伤(acute lung injury,ALI)的保护作用。方法健康雄性昆明种小鼠30只,随机分为5组,每组6只,即空白对照组、ALI组、低剂量治疗组、中剂量治疗组及高剂量治疗组。适应性饲养1周,在麻醉状态下用浓度为10 mg/m L的PM2.5混悬液进行气管滴注染毒,空白对照组用等量生理盐水代替,1/d,连续3 d。末次染毒24 h后,低、中、高剂量治疗组分别用浓度为10、20和40 mg/m L的壳低聚糖溶液灌胃,1/d,连续10 d,末次灌胃24 h后处死。分别检测肺组织匀浆和肺泡灌洗液中乳酸脱氢酶(lactate dehydrogenase,LDH)活性;肺组织匀浆中肿瘤坏死因子(tumor necrosis factor-α,TNF-α)含量;心脏取血检测血清超氧化物歧化酶(superoxide dismutase,SOD)活性;取肺组织作病理切片,HE染色进行形态学观察。结果与对照组比较,ALI组炎性渗出明显伴大量炎性细胞浸润,肺泡结构破坏融合,各治疗组中肺组织炎性渗出、炎性细胞浸润和肺泡结构破坏均减少;低、中、高剂量治疗组LDH活性、TNF-α含量均低于ALI组,SOD活性高于ALI组(P<0.01);且中剂量治疗组LDH活性、TNF-α含量降低和SOD活性增高效果更显著(P<0.01)。结论壳低聚糖对PM2.5所致的肺损伤有较好的保护作用。Objective To investigate the protective effect of chitosan oligosaccharides on PM2.5-induced acute lung injury(ALI).Methods Thirty healthy male Kunming mice were randomly divided into five groups,control group,ALI group,low-dose,medium-dose and high-dose treatment group,with 6 mice in each group.After a week of adaptive feeding,mice were treated by intratracheal instillation of normal saline or isovolumetric PM2.5 suspension(10 mg/m L)once a day for three consecutive days.24 hours after the final intratracheal instillation,the treatment groups were given chitosan with concentrations of 10 mg/m L,20 mg/m L,40 mg/m L respectively by gavage once a day for 10 consecutive days.The mice were sacrificed 24 hours after the last gavage.The activity of lactate dehydrogenase(LDH)both in lung homogenate and bronchoalveolar lavage fluid was detected,the level of tumor necrosis factor-α(TNF-α)in lung homogenate and activity of superoxide dismutase(SOD)in the blood were also detected.Additionally,histological alteration of lungs were examined by HE staining.Results Compared with the control group,the ALI group showed obvious inflammatory exudation in lungs with massive inflammatory cells and damage of alveolar structure.While in treatment groups,these lesious was relieved.Compared with those in ALI group,the level of TNF-αand the activity of LDH were lower,while the activity of SOD was higher in low-dose,medium-dose and high-dose treatment group(P<0.01).The decrease in LDH activity and TNF-αlevel as well as the increase in SOD activity of the medium-dose treatment group were more obvious(P<0.01).Conclusion The results indicate that chitosan oligosaccharides has a potential protective effect on PM2.5-induced lung injury in mice.

关 键 词：壳低聚糖 肺损伤 PM2.5 保护作用 

分 类 号：R965.1[医药卫生—药理学]