复合全氟三丁胺携氧材料的嗅鞘细胞移植促进大鼠周围神经损伤修复的研究  

Perfluorotributylamine oxygen-carrying material-olfactory ensheathing cell composition promote repair of sciatic nerve injury in rats

作　　者：雷可 雷涛 朱萌 邱容 王倩 梁卓文 LEI Ke;LEI Tao;ZHU Meng;QIU Rong;WANG Qian;LIANG Zhuowen(Department of Orthopedics,Xijing Hospital,Air Force Medical University,Xi'an Shaanxi 710032,China;Faculty of Military Biomedical Engineering,School of Basic Medical Sciences,Air Force Medical University,Xi'an Shaanxi 710032,China)

机构地区：[1]空军军医大学西京医院骨科,陕西西安710032 [2]空军军医大学基础医学院军事生物医学工程系,陕西西安710032

出　　处：《转化医学杂志》2020年第5期266-270,共5页Translational Medicine Journal

基　　金：国家自然科学基金青年科学基金(51907197)。

摘　　要：目的探究复合全氟三丁胺(perfluorotributylamine,PFTBA)的嗅鞘细胞(olfactory ensheathing cells,OECs)移植能否更进一步促进外周神经损伤后神经再生以及功能恢复。方法从大鼠嗅球分离并纯化OECs并鉴定。制作大鼠坐骨神经缺损模型。将60只坐骨神经缺损大鼠随机分为3组:自体神经移植组,OECs神经导管组,PFTBA-OECs神经导管组。在体内环境下分别测试术后3、7、14、28 d PFTBA对OECs存活率的影响。术后4周,对再生神经进行免疫荧光染色以及超薄切片,确定PFTBA-OECs对再生神经的作用。并在术后2、4、8周分别对各实验组大鼠坐骨神经功能指数值进行测量,以确定神经功能恢复情况。结果分离纯化的原代OECs纯度为(95.8±2.1)%;体内条件下,PFTBA能够大幅提高OECs的存活率(P<0.05);与OECs神经导管组比较,PFTBA-OECs神经导管组极大地促进了神经轴突的再生(P<0.05);再生神经透射电镜显示,PFTBA-OECs神经导管组再生神经轴突数量明显多于OECs神经导管组,髓鞘厚度明显优于OECs神经导管组(P<0.05);术后坐骨神经功能指数值显示,PFTBA-OECs神经导管组大鼠功能恢复情况明显优于OECs神经导管组(P<0.05)。结论 PFTBA能够明显提升OECs的促神经修复能力,明显提升神经损伤后的轴突再生能力以及髓鞘化程度,为解决细胞移植后面临的缺氧微环境提供了解决方案。Objective To investigate the effect of Perfluorotributylamine(PFTBA)on the promotion of olfactory ensheathing cells(OECs)in enhancing nerve regeneration.Methods The primary OECs were harvested from the olfactory bulb of rats and prepared as seeding cells.A model rat with sciatic nerve injury was made and sixty rats were randomly divided into 3 groups:the autograft,the OECs group and the PFTBA-OECs group.The effect of PFTBA on cell viability was examined at 3,7,14 and 28 d after surgery in vivo.The effect of PFTBA on axonal regeneration and axonal myelination was also examined by immunofluorescence staining and transmission electron microscope at 4 weeks after surgery.Furthermore,the effect of PFTBA on functional recovery after sciatic nerve injury was examined by evaluating the sciatic nerve function index(SFI)for each group after surgery at two,four or eight weeks.Results The purity of OECs was(95.8±2.1)%.The viability of OECs was significantly promoted by PFTBA in vivo(P<0.05).In addition,the immunofluorescence staining results showed that PFTBA-OECs significantly promoted nerve regeneration compared to OECs group(P<0.05).The transmission electron microscope results also showed that the number of myelinated axons and the thickness of myelin shealth in PFTBA-OECs were significantly superior to OECs group(P<0.05).Furthermore,the SFI values of each treatment showed that PFTBA-OECs significantly promoted the functional recovery after nerve injury compared to OECs groups(P<0.05).Conclusion PFTBA significantly promoted the cell viability and the capacity of transplanted OECs,enhancecl nerve repair,which provided a strategy to improve the hypoxia environment of transplanted cells.

关 键 词：嗅鞘细胞 全氟三丁胺 周围神经损伤 缺氧 神经再生 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]