体外CM-DIL标记骨髓源性内皮祖细胞在胶原海绵上的研究  

A Vitro Study on CM-DIL Labeling of Bone Marrow Derived Endothelial Progenitor Cells in Collagen Sponge

作　　者：韩刘君 孙尧 于晓龙 谷玉 Han Liujun;Sun Yao;Yu Xiaolong;Gu Yu(Department of Geriatrics,North China University of Science and Technology,Tangshan 063000,China;不详)

机构地区：[1]华北理工大学附属医院老年病科,唐山063000

出　　处：《中国循证心血管医学杂志》2020年第9期1067-1070,1074,共5页Chinese Journal of Evidence-Based Cardiovascular Medicine

基　　金：河北省优秀人才基金[冀财社(2017)46]。

摘　　要：目的观察CM-DIL体外标记兔骨髓来源的内皮祖细胞(EPCs)的可行性及其在胶原海绵上的生长情况。方法用Percoll分离液的方法分离、培养EPCs,并进行鉴定;观察CM-DIL标记后的EPCs与胶原海绵的生物相容性,HE染色评价EPCs的生长情况。结果CM-DIL标记EPCs的细胞膜为红色,分离培养的EPCs其表面标志物CD31和CD34表达均为阳性,种植于胶原海绵上的EPCs可存活,并沿胶原海绵支架生长。结论CM-DIL可作为体外标记EPCs的方法,胶原海绵可作为一种较为理想的组织工程学支架材料。Objective To observe the feasibility of CM-DIL labeling endothelial progenitor cells(EPCs)derived from rabbit bone marrow in vitro and its growth on collagen sponge.Methods Percoll separation method was used to isolate,culture and identify EPCs;the biocompatibility of CM-DIL labeled EPCs and collagen sponge was observed,and the growth of EPCs was evaluated by HE staining.Results The cell membrane of CM-DIL labeled EPCs was red.The isolated and cultured EPCs expressed positive surface markers CD31 and CD34.The EPCs planted on collagen sponge can survive and grow along the collagen sponge scaffold.Conclusion CM-DIL can be used as a method for labeling EPCs in vitro,and collagen sponge can be used as an ideal tissue engineering scaffold material.

关 键 词：内皮祖细胞 CM-DIL 胶原海绵 兔 

分 类 号：R5[医药卫生—内科学]