温针灸对慢性萎缩性胃炎大鼠局部穴区组织代谢的影响  

Effects of Warm Acupuncture on Tissue Metabolism of Local Acupoints of Rats with Chronic Atrophic Gastritis

作　　者：曹佳男 钟欢[1] 刘密[1,2] 罗晓婷 王英姿[3] 常小荣[1] 冯芳[3] CAO Jianan;ZHONG Huan;LIU Mi;LUO Xiaoting;WANG Yingzi;CHANG Xiaorong;FENG Fang(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Liuyang City Traditional Chinese Medicine Hospital,Liuyang,Hunan 410300,China;West Hospital of Chenzhou First People's Hospital,Chenzhou,Hunan 423000,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]浏阳市中医医院,湖南浏阳410300 [3]郴州市第一人民医院西院,湖南郴州423000

出　　处：《湖南中医药大学学报》2020年第9期1054-1059,共6页Journal of Hunan University of Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2015CB554502);国家自然科学基金项目(81574082);郴州市科技发展计划项目(ZDYF2020098)。

摘　　要：目的基于核磁共振氢谱(1H nuclear magnetic resonance spectra,1H-NMR)代谢组学技术,观察温针灸对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠局部穴区组织代谢的影响,从代谢物的差异性分析,以探究温针灸治疗CAG可能的作用机制。方法42只大鼠随机分为正常组、模型组、模型+温针灸组,每组14只,正常组正常喂养,模型组、模型+温针灸组给予造模处理,造模12周后,每组各取2只处死,用于模型评价,造模成功后分别按各组受试因素干预。HE染色观察大鼠胃组织病理学变化评价造模情况以及温针灸干预后的病理变化,1H-NMR技术检测大鼠局部穴区组织的代谢物,用Chenomx NMR Suite软件分析所得数据。结果模型组大鼠胃组织病理符合CAG病理诊断标准,模型+温针灸组干预后的大鼠胃组织病理变化有一定程度改善。温针灸可回调CAG大鼠局部穴区组织乳酸、N,N-二甲基甘氨酸、肌醇、一磷酸腺苷、腺苷、次黄嘌呤的含量,下降甜菜碱、苏氨酸、磷酸胆碱、甘磷酸胆碱、二磷酸腺苷、肌苷的含量。结论从代谢的角度证实了温针灸对CAG大鼠有向愈性调整,温针灸主要调节大鼠的核酸及能量代谢。一磷酸腺苷、次黄嘌呤、肌苷、磷酸胆碱等可能是温针灸治疗CAG的代谢物质基础。Objective To observe the effect of warm acupuncture on tissue metabolism of local acupoints in rats with chronic atrophic gastritis(CAG)based on 1H nuclear magnetic resonance spectra(1H-NMR),and to explore the possible mechanism of warm acupuncture in the treatment of CAG through analysis of difference in metabolite.Methods A total of 42 rats were randomly divided into a normal group,a model group and a model+warm acupuncture group,with 14 rats in each group.The normal group was fed normally.The model group and the model+warm acupuncture group were treated with modeling.After 12 weeks of modeling,2 rats in each group were killed for model evaluation.After successful modeling,intervention was conducted according to the test factors in each group.He staining was used to observe the pathological changes of gastric tissue in rats,and the pathological changes after warm needle moxibustion intervention were evaluated.1H-NMR was used to detect the metabolites in the local acupoint area of rats.The data were analyzed by chenomx NMR suite software.Results The pathological changes of gastric tissue in the model group were in accordance with CAG pathological diagnostic criteria,and the pathological changes of gastric tissue in the model+warm acupuncture group were improved to some extent.Moreover,warm acupuncture can reverse the contents of lactic acid,N,N-Dimethylglycine,inositol,adenosine monophosphate,adenosine,hypoxanthine,and decrease the contents of betaine,threonine,phosphorylcholine,choline alfoscerate,adenosine diphosphate and inosine.Conclusion From the point of view of metabolism,it is confirmed that warm acupuncture has a healing effect on CAG rats.Warm acupuncture mainly regulates nucleic acid metabolism and energy metabolism in rats.adenosine monophosphate,hypoxanthine,inosine and choline phosphate may be the metabolic basis of warm acupuncture treatment of CAG.

关 键 词：慢性萎缩性胃炎 针刺 温针灸 穴区组织 代谢组学 

分 类 号：R245[医药卫生—针灸推拿学]