miR-93-3p对高糖诱导的肾小管上皮细胞损伤及炎症因子分泌的影响  

miR-93-3p inhibits high glucose-induced renal tubular epithelial cell damage and inflammatory factor secretion via PI3K/AKT signaling pathway

作　　者：李锴[1] 朱志辉 马医林 唐军涛 林园园 杨小娟[3] 安智[3] LI Kai;ZHU Zhihui;MA Yilin;TANG Juntao;LIN Yuanyuan;YANG Xiaojuan;AN Zhi(Department of Endocrinology and Nephrology,Hanzhong People's Hospital,Hanzhong 723000,Shanxi,China;Department of Nephropathy,Mian County Hospital,Mian County 724206,Shanxi,China;Department of Nephrology,Yan'an University Affiliated Hospital,Yan'an 716000,Shanxi,China)

机构地区：[1]汉中市人民医院内分泌肾脏病科,陕西汉中723000 [2]勉县县医院肾病内科,陕西勉县724206 [3]延安大学附属医院肾内科,陕西延安716000

出　　处：《西部医学》2020年第10期1420-1424,1431,共6页Medical Journal of West China

基　　金：陕西省科学技术项目(2016-01-0131)。

摘　　要：目的探讨微小miRNA-93-3p(miR-93-3p)对高糖诱导的肾小管上皮细胞损伤及炎症因子分泌的影响。方法体外培养肾小管上皮细胞,分为NC组、HG组、anti-miR-NC组、anti-miR-93-3p组、HG+miR-NC组、HG+miR-93-3p组。采用实时荧光定量聚合酶链反应(qRT-PCR)检测miR-93-3p的表达;酶联免疫吸附(ELISA)法检测肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)水平;甲基噻唑基四唑(MTT)检测细胞增殖能力;流式细胞术检测细胞凋亡率;蛋白免疫印迹法(Western blot)检测细胞周期蛋白1(CyclinD1)、活化的含半胱氨酸的天冬氨酸蛋白水解酶3(C-caspase-3)、磷酸化-磷脂酰肌醇激酶(p-PI3K)、磷酸化-蛋白激酶B(p-AKT)表达量。结果与NC组相比,HG组miR-93-3p的表达水平显著降低(P<0.05),细胞存活率显著降低(P<0.05),CyclinD1蛋白表达水平显著降低(P<0.05),而细胞凋亡率显著升高(P<0.05),C-caspase-3、p-PI3K、p-AKT蛋白表达水平显著升高(P<0.05),TNF-α、IL-6水平显著升高(P<0.05);与anti-miR-NC组相比,anti-miR-93-3p组细胞存活率显著降低(P<0.05),细胞凋亡率显著升高(P<0.05),C-caspase-3蛋白表达水平显著升高(P<0.05),TNF-α、IL-6水平显著升高(P<0.05);与HG+miR-NC组相比,HG+miR-93-3p组细胞存活率显著升高(P<0.05),细胞凋亡率显著降低(P<0.05),CyclinD1蛋白表达水平显著升高(P<0.05),C-caspase-3、p-PI3K、p-AKT蛋白表达水平显著降低(P<0.05),TNF-α、IL-6水平显著降低(P<0.05)。结论miR-93-3p通过调控PI3K/AKT信号通路抑制高糖诱导的肾小管上皮细胞损伤及炎症因子分泌。Objective To investigate the effects of microRNA-93-3 p(miR-93-3 p)on high glucose-induced renal tubular epithelial damage,inflammatory factor secretion and PI3 K/AKT signaling pathway.Methods Renal tubular epithelial cells were cultured in vitro and divided into NC group,HG group,anti-miR-NC group,anti-miR-93-3 p group,HG+miR-NC group,HG+miR-93-3 p group.Renal tubular epithelial cells were cultured in vitro,and the experimental groups were:NC group,HG group,anti-miR-NC group,anti-miR-93-3 p group,HG+miR-NC group,HG+miR-93-3 p group.The expression of miR-93-3 p was detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).The levels of TNF-αand IL-6 were detected by enzyme-linked immunosorbent assay(ELISA).MTT was used to detect cell proliferation ability.Apoptosis rate was measured by flow cytometry.The expression levels of CyclinD1,C-caspase-3,p-PI3 K and p-AKT were detected by Western blot.Results Compared with the NC group,the expression level of miR-93-3 p was significantly decreased in the HG group(P<0.05),the cell survival rate was significantly decreased(P<0.05),and the expression level of CyclinD1 was significantly decreased(P<0.05),but the apoptosis rate was significantly increased(P<0.05),the expression levels of C-caspase-3,p-PI3 K and p-AKT were significantly increased(P<0.05),and the levels of TNF-αand IL-6 were significantly increased(P<0.05).<0.05).Compared with the anti-miR-NC group,the cell survival rate of the anti-miR-93-3 p group was significantly decreased(P<0.05),and the expression level of CyclinD1 was significantly decreased(P<0.05),but the apoptosis rate was significantly increased(P<0.05),the expression level of C-caspase-3 was significantly increased(P<0.05),and the levels of TNF-αand IL-6 were significantly increased(P<0.05).Compared with HG+miR-NC group,the survival rate of HG+miR-93-3 p group was significantly increased(P<0.05),the apoptosis rate was significantly decreased(P<0.05),and the expression level of CyclinD1 was significantly increased(P<0.05).<0

关 键 词：miR-93-3p 高糖 肾小管上皮细胞 PI3K/AKT 炎症 凋亡 

分 类 号：R587.2[医药卫生—内分泌]