林下参内生球毛壳菌FS-01对人参病原真菌的抑制作用  

Inhibitory effect of Chaetomium globosum FS-01 from mountain-cultivated ginseng against ginseng pathogens

作　　者：周春元[1] 许世泉[1] 闫梅霞[1] 崔丽丽[1] 华霜 王英平[1] ZHOU Chunyuan;XU Shiquan;YAN Meixia;CUI Lili;HUA Shuang;WANG Yingping(Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《广西植物》2020年第9期1341-1348,共8页Guihaia

基　　金：国家重点研发计划课题(2017YFC1702102);吉林省自然科学基金(20170101011JC);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)。

摘　　要：为探究林下参内生真菌球毛壳菌(Chaetomium globosum)FS-01菌株对人参病原菌的抑菌作用,该研究在实验室条件下,测定了FS-01菌株菌丝、发酵液和孢子悬浮液对人参黑斑病菌(Alternaria panax)、人参菌核病菌(Sclerotinia schinseng)、人参灰霉病菌(Botrytis cinerea)、人参立枯病菌(Rhizoctonia solani)、人参根腐病菌(Fusarium solani)5种人参病原菌的抑制作用。结果表明:内生真菌球毛壳菌FS-01对5种病原菌均有抑制作用,其中,对人参黑斑病菌的抑制作用最高,为30.80%,其次是人参立枯病菌、人参菌核病菌、人参根腐病菌和人参灰霉病菌;发酵液抑菌实验结果表明,在加入内生真菌球毛壳菌FS-01菌株发酵液的PDA培养基上,对人参灰霉病菌的抑制作用最高,为82.09%,其次是人参菌核病菌、人参黑斑病菌、人参立枯病菌和人参根腐病菌;孢子抑菌实验结果表明,在加入内生真菌球毛壳菌FS-01菌株孢子悬浮液的PDA培养基上,对人参黑斑病菌的抑制作用最高,为83.72%,其次是人参灰霉病菌、人参立枯病菌、人参菌核病菌和人参根腐病菌。综上结果认为,内生真菌球毛壳菌FS-01菌株对人参病原菌均有很高的抑菌作用,可作为人参病原菌的生防菌株资源。To study the inhibitory effects of Chaetomium globosum strain FS-01 against Alternaria panax,Sclerotinia schinseng,Botrytis cinerea,Rhizoctonia solani and Fusarium solani of ginseng pathogens,mycelia,fermentation broth and conidia suspension were tested in laboratory.The results showed that Chaetomium globosum FS-01 had certain inhibitory effects against five kinds of ginseng pathogens by confront culture.Among them,the highest inhibition rate to mycelia growth of Alternaria panax,reached 30.80%,followed by Rhizoctonia solani,Sclerotinia schinseng,Fusarium solani and Botrytis cinerea;On the PDA plate mixed with Chaetomium globosum FS-01 fermentation broth,the highest inhibition rate to mycelia growth of Botrytis cinerea,reached 82.09%,followed by Sclerotinia schinseng,Alternaria panax,Rhizoctonia solani and Fusarium solani;On the PDA plate mixed with Chaetomium globosum FS-01 conidia suspension,the highest inhibition rate to mycelia growth of Alternaria panax,reached 83.72%,followed by Botrytis cinerea,Rhizoctonia solani,Sclerotinia schinseng and Fusarium solani.Endophytic fungi FS-01 strain had a significant inhibitory effects,it could be utilized as a biological control resource inhibiting ginseng pathogens.

关 键 词：林下参 内生真菌 球毛壳菌 人参病原菌 抑制作用 

分 类 号：Q945.8[农业科学—植物病理学]