脊尾白虾低盐转录组信息挖掘  

Mining of Information in Transcriptome of Exopalaemon carinicauda under Low Salinity Stress

作　　者：沈晔 王兴强 曹梅 郑年昊 陈百尧 SHEN Ye;WANG Xing-qiang;CAO Mei(College of Marine Life and Fisheries,Jiangsu Ocean University,Lianyungang,Jiangsu 222005)

机构地区：[1]江苏海洋大学海洋生命与水产学院,江苏连云港222005 [2]连云港市农业农村局,江苏连云港222005

出　　处：《安徽农业科学》2020年第19期89-96,共8页Journal of Anhui Agricultural Sciences

基　　金：江苏省研究生科研与实践创新计划项目(SJCX18_0927);江苏省海洋生物技术重点实验室开放基金项目(HS16005);连云港市“海燕计划”科研项目。

摘　　要：通过前期对自然海水组(盐度31.0)和淡水组(盐度0.2)脊尾白虾(Exopalaemon carinicauda)转录组的测序及分析,筛选出低盐胁迫下差异表达显著的基因并进行实时定量验证,同时进行KEGG通路富集分析,挖掘低盐胁迫相关信号通路,以期阐明脊尾白虾在低盐环境下的生理调控机制。转录组数据分析发现,脊尾白虾机体可能通过磺基转移酶活性的变化来调控类固醇、激素水平。表皮蛋白AMP5样的表达量的变化表明低盐胁迫可能影响脊尾白虾节间膜的形成。低盐胁迫试验实时定量结果显示,基因在鳃、肝胰腺和肌肉中的表达情况各有不同,并随时间的变化,基因的表达量也会有所波动。Hedgehog信号通路分析发现,低盐度胁迫条件下脊尾白虾C型凝集素和低密度脂蛋白受体相关蛋白表达上调,推测机体通过调控C型凝集素和低密度脂蛋白受体相关蛋白,促进低密度脂蛋白的代谢,水解得到的胆固醇与Smo结合,激活Hedgehog信号通路,以响应低盐胁迫。该研究挖掘了差异表达基因相关信号通路,推测低盐胁迫可能通过调控代谢相关酶的活性来促进脊尾白虾的生长代谢,并且低盐胁迫可能影响神经递质的调控过程。By sequencing and analyzing the transcriptome of Exopalaemon carinicauda in natural seawater(salinity 31.0)and fresh water(salinity 0.2)in the early stage,we screened out the genes with significant differential expression under low salt stress and carried out real-time quantitative verification.At the same time,the enrichment analysis of KEGG pathway was carried out to explore the signal pathway related to low salt stress,in order to clarify the physiological regulation mechanism of E.carinicauda in low salt environment.The results of transcriptome analysis showed that E.carinicauda might regulate steroid and hormone levels by increasing the activity of sulfotransferase.The down-regulation of amp5-like expression of epidermal protein suggests that low salt stress might affect the formation of internode membrane.The real-time quantitative results of low salt stress test showed that the expression of genes in gill,hepatopancreas and muscle were different,and with the change of time,the expression of genes would fluctuate.The analysis of hedgehog signaling pathway showed that the C-type lectin and LDL receptor related proteins were up-regulated under low salinity stress.It was speculated that the body could promote the metabolism of LDL by regulating the C-type lectin and LDL receptor related proteins,and the hydrolyzed cholesterol binds to Smo,thus activating hedgehog signaling pathway in response to low salt stress.In this study,we explored the differentially expressed gene related signal pathways,and speculated that low salt stress might promote the growth and metabolism by regulating the activity of metabolism related enzymes,and low salt stress might affect the regulation process of neurotransmitters.

关 键 词：脊尾白虾 低盐胁迫 转录组 

分 类 号：Q786[生物学—分子生物学]