瑶药满山香总黄酮提取工艺的优化  

Optimization of extraction technology of total flavonoids from Chinese medicine Schisandra propinqua

作　　者：唐春丽[1] 魏江存 陆梅元 谢臻 陈宏夏 赵凤仙 TANG Chun-li;WEI Jiang-cun;LU Mei-yuan;XIE Zhen;CHEN Hong-xia;ZHAO Feng-xian(The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China;Guangxi International Zhuang Medical Hospital,Nanning 530201,China;Guangxi District Food and Drug Review and Inspection Center,Nanning 530029,China;School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学第一附属医院,南宁530023 [2]广西国际壮医医院,南宁530201 [3]广西壮族自治区食品药品审评查验中心,南宁530029 [4]广西中医药大学药学院,南宁530200

出　　处：《湖北农业科学》2020年第16期126-129,138,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(81360524);2019年度广西高校中青年教师科研基础能力提升项目(2019KY0341);广西中医药大学青年基金项目(2019QN036);广西自然科学基金项目(2014GXNSFAA118208);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室(国中医药发2009[21]号)。

摘　　要：采用回流提取法提取满山香(Schisandra propinqua)总黄酮,通过单因素试验,考察提取方法、提取溶剂体积分数、提取液体积、提取时间对药材总黄酮含量的影响,采用正交试验,优化提取工艺条件,筛选出满山香总黄酮的最佳提取工艺。结果表明,满山香总黄酮的最佳提取工艺为60%乙醇、料液比1∶40(m∶V)、回流提取时间1.0 h。该方法能有效测定满山香药材总黄酮的含量,重复性较好、稳定可行。The total flavonoids of Schisandra propinqua were extracted by reflux extraction method.The effects of extraction method,extraction solvent volume fraction,extraction volume and extraction time on the total flavonoids content of the medicinal materials were investigated by single factor experiment.The orthogonal test was used to optimize the extraction conditions.The best extraction process for a total flavonoid of Schisandra propinqua was screened out.The results showed that the optimal extraction process of a total flavo⁃noid of Schisandra propinqua was 70%methanol,the ratio of material to liquid was 1∶40(m∶V),and the reflux extraction time was 1.0 h.This method could effectively determine the content of total flavonoids in Schisandra propinqua medicinal material,the repeatability was good,the method was stable and feasible.

关 键 词：满山香(Schisandra propinqua) 总黄酮 提取工艺 

分 类 号：R282[医药卫生—中药学]