Agrobacterium-mediated delivery of CRISPR/Cas reagents for genome editing in plants enters an era of ternary vector systems  

作　　者：Yu Zhang Qiang Zhang Qi-Jun Chen 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,China [2]Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing 100193,China

出　　处：《Science China(Life Sciences)》2020年第10期1491-1498,共8页中国科学（生命科学英文版）

基　　金：Supported by grants from the National Crop Breeding Fund (2016YFD0101804);the National Natural Science Foundation of China (31872678 and 31670371);and the National Transgenic Research Project (2016ZX08009002).

摘　　要：Lack of appropriate methods for delivery of genome-editing reagents is a major barrier to CRISPR/Cas-mediated genome editing in plants.Agrobacterium-mediated genetic transformation(AMGT)is the preferred method of CRISPR/Cas reagent delivery,and researchers have recently made great improvements to this process.In this article,we review the development of AMGT and AMGT-based delivery of CRISPR/Cas reagents.We give an overview of the development of AMGT vectors including binary vector,superbinary vector,dual binary vector,and ternary vector systems.We also review the progress in Agrobacterium genomics and Agrobacterium genetic engineering for optimal strains.We focus in particular on the ternary vector system and the resources we developed.In summary,it is our opinion that Agrobacterium-mediated CRISPR/Cas genome editing in plants is entering an era of ternary vector systems,which are often integrated with morphogenic regulators.The new vectors described in this article are available from Addgene and/or MolecularCloud for sharing with academic investigators for noncommercial research.

关 键 词：genome editing CRISPR/Cas ternary vector system delivery Agrobacterium-mediated transformation 

分 类 号：Q943.2[生物学—植物学]