Expression patterns and functional divergence of homologous genes accompanied by polyploidization in cotton(Gossypium hirsutum L.)  

作　　者：Zhen Peng Hua Cheng Gaofei Sun Zhaoe Pan Xiao Wang Xiaoli Geng Shoupu He Xiongming Du 

机构地区：[1]Research Base,Anyang Institute of Technology,State Key Laboratory of Cotton Biology/Institute of Cotton Research,Chinese Academy of Agricultural Science(ICR,CAAS),Anyang 455000,China [2]Zhengzhou Research Base,State Key Laboratory of Cotton Biology,Zhengzhou University,Zhengzhou 450001,China [3]Anyang Institute of Technology,Anyang 455000,China

出　　处：《Science China(Life Sciences)》2020年第10期1565-1579,共15页中国科学（生命科学英文版）

基　　金：Funded by the National Key Research and Development Program of China(2016YFD0100203 and 2016YFD0100306);the Foundation and Frontier Research Grant of Henan Provincial Science and Technology Bureau(162300410171).

摘　　要：Naturally allotetraploid cotton has been widely used as an ideal model to investigate gene expression remodeling as a consequence of polyploidization.However,the global gene pattern variation during early fiber development was unknown.In this study,through RNA-seq technology,we comprehensively investigated the expression patterns of homologous genes between allotetraploid cotton(G.hirsutum)and its diploid progenitors(G.arboreum and G.raimondii)at the fiber early development stage.In tetraploid cotton,genes showed expression level dominance(ELD)bias toward the A genome.This phenomenon was explained by the up-/downregulation of the homologs from the nondominant progenitor(D genome).Gene ontology(GO)enrichment results indicated that the ELD-A genes might be a prominent cause responsible for fiber property change through regulating the fatty acid biosynthesis/metabolism and microtubule procession,and the ELD-D genes might be involved in transcription regulation and stress inducement.In addition,the number and proportion of completely A-and D-subfunctionalized gene were similar at different fiber development stages.However,for neofunctionalization,the number and proportion of reactivated D-derived genes were greater than those of A at 3 and 5 DPA.Eventually,we found that some homologous genes belonging to several specific pathways might create novel asymmetric transcripts between two subgenomes during polyploidization and domestication process,further making the fiber property meet the human demands.Our study identified determinate pathways and their involved genes between allotetraploid cotton and their progenitors at early fiber development stages,providing new insights into the mechanism of cotton fiber evolution.

关 键 词：GOSSYPIUM fiber development polyploid evolution expression level dominance sub-and neofunctionalization 

分 类 号：S562[农业科学—作物学]