不同类型心脏停搏液对小鼠未成熟心肌细胞增殖能力的影响  

Effects of different types of cardioplegia on the proliferation of immature cardio⁃myocytes of newborn mice

作　　者：刘怀普[1] 梁彦锴 吴柯叶 郑丰楠 谭小莉[1] 孟保英[1] Liu Huaipu;Liang Yankai;Wu Keye;Zheng Fengnan;Tan Xiaoli;Meng Baoying(Department of Cardiothoracic Surgery,Shenzhen Children’s Hospital,Shenzhen518038,China)

机构地区：[1]深圳市儿童医院心胸外科,深圳518038

出　　处：《中国体外循环杂志》2020年第5期307-311,320,共6页Chinese Journal of Extracorporeal Circulation

基　　金：深圳市科创委基础研究自由探索项目(JCYJ2017030315567876)。

摘　　要：目的观察不同类型心脏停搏液对离体新生小鼠未成熟心肌细胞增殖能力的影响。方法体外分离培养新生小鼠心肌细胞,随机分为5组,分别给予复方电解质液、改良圣托马斯液(STH)、HTK液以及仿del Nido液干预2 h后,继续培养24~72 h,分别采用EdU荧光染色、Ki-67荧光染色及MTT比色法观察细胞增殖情况;采用Western Blot免疫印迹法检测YAP1蛋白表达水平。结果EdU荧光染色和Ki-67免疫组织化学染色阳性细胞计数:HTK液组最高,改良STH液组次之,仿del Nido液低于复方电解质组;MTT法检测OD值:HTK液组最高,改良STH液组次之,仿del Nido液高于复方电解质组;YAP1 Western Blot灰度值:HTK液组高于其他各组。结论HTK液和改良STH液对离体小鼠未成熟心肌增殖能力具有明显的保护作用。Objective To observe the effects of different types of cardioplegia on the proliferation of immature cardiomyocytes of newborn mice in vitro.Methods Cardiomyocytes of newborn mice were isolated and cultured in vitro and randomly divided into 5 groups.After 2 hours intervention with compound electrolyte solution,cardiomyocytes were further cultured for 24-72 hours.The prolif⁃eration of cardiomyocytes was observed by technique of EdU fluorescence staining,Ki-67 fluorescence staining and MTT colorimetry.The expression of YAP1 protein was detected by Western blot.Results EdU staining and Ki-67 immunopositive cell count:The HTK group was the highest,followed by the modified STH group,and the del Nido analogue group was lower than the compound electrolyte group.OD value detected by MTT method:The HTK group was the highest,followed by the modified STH,and the del Nido analogue group was higher than the compound electrolyte group.YAP1 western blot gray value of HTK group was higher than other groups.Con⁃clusion HTK solution and modified STH solution have significant protective effects on the proliferation of immature cardiomyocytes of newborn mice in vitro.

关 键 词：心脏停搏液 未成熟心肌细胞 细胞增殖 心肌保护 小鼠 

分 类 号：R654.2[医药卫生—外科学] R-332[医药卫生—临床医学]