Effects of Vitrification on the Imprinted Gene Snrpn in Neonatal Placental Tissue  

作　　者：Jian-Feng Yao Yan-Fang Huang Rong-Fu Huang Su-Xia Lin Cai-Qiong Guo Cheng-Zhou Hua Pei-Ya Wu Ji-Feng Hu You-Zhu Li 

机构地区：[1]Center for Reproductive Medicine,Quanzhou Maternity and Child Healthcare Hospital,Quanzhou 362000,China [2]Health Screening Center,The First Affiliated Hospital,Fujian Medical University,Fuzhou 350004,China [3]Clinical Testing Center,The Second Affiliated Hospital,Fujian Medical University,Quanzhou 362000,China [4]Department of Obstetrics and Gynecology,The First Affiliated Hospital,Fujian Medical University,Fuzhou 350004,China [5]Center for Reproductive Medicine,The First Affiliated Hospital of Xiamen University,Xiamen 361003,China

出　　处：《Reproductive and Developmental Medicine》2020年第1期25-31,共7页生殖与发育医学（英文版）

基　　金：supported by the Quanzhou Science and Technology Plan Project in 2016(No.2016Z38);the Natural Science Foundation Science and Technology Project Plan of Fujian in 2018(No.2018J01147).

摘　　要：Objective:To investigate the effects of vitrification on the expression of the imprinted gene Snrpn in neonatal placental tissue.Methods:Neonatal placental tissue was collected from women with natural pregnancy(control group)and from women in assisted reproductive technology(ART)pregnancy group,following fresh and vitrified embryo transfer(fresh group and vitrified group,respectively).Snrpn mRNA expression and SNRPN protein levels in placental tissue from these three groups were assessed by real-time reverse transcription polymerase chain reaction and Western blot,respectively.DNA methylation in the Snrpn promoter region was analyzed by bisulfite-pyrosequencing.Results:The expression of Snrpn mRNA and SNRPN protein was found to be higher in placental tissue from the fresh and vitrified ART groups,compared to the control group.There was no significant difference in SNRPN gene or protein expression between the fresh and vitrified groups.DNA methylation at the Snrpn promoter region was not significantly different between these three groups.Conclusions:Human ART may alter the transcriptional expression and protein levels of the imprinted gene Snrpn.However,compared to other ART methods,vitrification may not aggravate or reduce this effect.Moreover,the altered expression of Snrpn is likely not directly related to DNA methylation of the Snrpn promoter region.

关 键 词：Assisted Reproductive Technology Polymerase Chain Reaction SNRPN VITRIFICATION Western Blot 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] Q34[医药卫生—基础医学]