土生隐球酵母在铝胁迫下钙调磷酸酶信号调控基因的转录组分析  

Transcriptome Analysis of Calcineurin Signal-regulated Genes in Cryptococcus humicola under Aluminum Stress

作　　者：代梦瑶 刘嘉 孔蕾 文增叶 谭勇[1] 年洪娟[1] Dai Mengyao;Liu Jia;Kong Lei;Wen Zengye;Tan Yong;Nian Hongjuan(Faculty of Life Sciences and Technology,Kunming University of Science and Technology,,Kunming,650500)

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500

出　　处：《基因组学与应用生物学》2020年第7期3052-3059,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31560246)资助。

摘　　要：钙调素-钙调磷酸酶信号通路是酵母细胞中重要的铝胁迫应答通路,在铝胁迫的条件下可激活钙调素和钙调磷酸酶的转录和翻译。之前的研究表明钙调磷酸酶能够参与铝胁迫下土生隐球酵母的生长,添加了铝的培养基上再添加FK506(钙调磷酸酶抑制剂)时会对土生隐球酵母的生长产生明显抑制,而未添加FK506的培养基上土生隐球酵母可以正常生长,只是菌落较小。为了研究钙调磷酸酶在土生隐球酵母耐铝过程中的作用,本研究提取土生隐球酵母RNA后,反转录得到cDNA文库,并利用二代高通量测序平台Illumina HiSeqTM2000对土生隐球酵母转录组进行测序。本研究初步表明,土生隐球酵母通过激活钙调磷酸酶途径抵抗铝胁迫。通过土生隐球酵母转录组分析得到的调控基因、结构基因以及差异表达基因,为进一步研究微生物耐铝中关键酶基因的挖掘、克隆和功能的验证提供了宝贵数据,为微生物耐铝机制的研究提供科学依据。Calmodulin-calcineurin signaling pathway is an important stress response pathway in yeast cells.Under the condition of aluminum stress,the transcription and translation of calmodulin and calcineurin can be activated.Previous studies have shown that calcineurin can participate in the growth of Cryptococcus humicola under aluminum stress.Adding calcineurin inhibitor to the medium containing aluminum significantly inhibited the growth of C.humicola indigenous.On the medium without FK506,C.humicola can grow normally,just a small colony.To study the role of calcineurin in the aluminum tolerance of C.humicola.After extracting the C.humicola’s RNA,the cDNA library was reverse transcribed,and the transcriptome of the C.humicola was sequenced using the second-generation high-throughput sequencing platform Illumina Hi SeqTM2000.The preliminary findings of this study indicate that,C.humicola resists against aluminum stress by activating calcineurin pathway.The regulatory genes,structural genes and differentially expressed genes obtained from transcriptome analysis of C.humicola provide valuable data for further study on the excavation,cloning and functional verification of key enzyme genes in aluminum tolerance of microorganisms,and lay a foundation for the study of aluminum tolerance mechanism of microorganisms.

关 键 词：土生隐球酵母 钙调磷酸酶通路 转录组分析 差异表达 

分 类 号：Q933[生物学—微生物学]