缺磷培养下黑木相思苗木的生理生化响应  

The Physiological and Biochemical Responses of Acacia melanoxylon Under Phosphorus Deficiency

作　　者：裘珍飞[1] 范春节[1] 曾炳山[1] Qiu Zhenfei;Fan Chunjie;Zeng Bingshan(Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou Guangdong 510520,China)

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《西南林业大学学报（自然科学）》2020年第6期27-33,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项资金重点项目(CAFYBB2018ZB004)资助。

摘　　要：采用黑木相思组培苗,在缺磷的Hoagland’s营养液下培养3个月,以全磷处理为对照,分析缺磷条件对黑木相思苗木生长、养分含量及其相关生理生化的影响,揭示黑木相思磷胁迫的响应机制。结果表明:缺磷培养后,黑木相思苗木株高、苗杆直径和叶片数量显著下降,但总根长显著增加;叶和根中超氧化物歧化酶、过氧化物酶和过氧化氢酶活性显著升高,其中叶中过氧化物酶和过氧化氢酶活性升高113.7%和473.3%;缺磷导致叶绿素含量、净光合速率降低,最大净光合速率和表观量子效率显著下降;化学组分分析中,缺磷显著降低了根、茎和叶器官中全氮、全磷和全钾元素含量,根和茎中全磷含量降低了82.9%和86.8%,而各器官的养分利用效率显著提高。通过3个月的缺磷培养,黑木相思苗木虽然生长量受到限制,但尚未出现典型的缺磷病症。To investigate the responses mechanism of phosphorus(P)deficiency stress,the plant growth,nutrient content and physiological and biochemical analysis were performed in Acacia melanoxylon.In this study,A.melanoxylon seedlings were cultivated in Hoagland’s media without P for 3 months and the Hoagland’s media was as the control.Results showed that plant height,stem diameter and number of leaves significantly decreased while the total root length significantly increased under P depletion.The superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities were increased in leaves and roots,among which the POD and CAT activity in leaf increased by 113.7%and 473.3%,respectively.Meanwhile,chlorophyll content and net photosynthetic rate(A),light-saturated photosynthesis(Amax)and apparent quantum yield(AQY)also decreased under P deficiency.Besides,nitrogen,phosphorus and potassium in root,stem and leaf also significantly decreased.Interestingly,the P contents in root and stem decreased by 82.9%and 86.8%,respectively.However,nutrient use efficiency indifferent organ showed significantly increased.In our study,we found that A.melanoxylon seedlings have not showed typical P deficiency phenotype although its growth was limited under 3 months of P deprivation.

关 键 词：黑木相思 苗木 磷胁迫 形态 生理生化 

分 类 号：Q945[生物学—植物学]