‘枸头’橙针刺和枝的转录组分析  

Transcriptome Analyses of the Thorn and Branch from ’Goutou’ Sour Orange

作　　者：孙立方 柯甫志[1] 聂振朋[1] 王平[1] 孙建华[1] 黄秀 徐建国[1] Sun Lifang;Ke Fuzhi;Nie Zhenpeng;Wang Ping;Sun Jianhua;Huang Xiu;Xu Jianguo(Zhejiang Branch of National Center for Citrus Variety Improvement,Citrus Research Institute of Zhejiang Academy of Agricultural Sciences,Taizhou,318026)

机构地区：[1]浙江省柑橘研究所,国家柑橘品种改良中心浙江分中心,台州318026

出　　处：《分子植物育种》2020年第19期6290-6297,共8页Molecular Plant Breeding

基　　金：国家现代农业(柑桔)产业技术体系岗位科学家项目(CARS-26);浙江省农业(果品)新品种选育重大科技专项(2016C2052-1);浙江省农业科学院扶持学科(柑橘育种)项目;台州优势水果产业技术创新团队项目共同资助。

摘　　要：柑橘枝干多具针刺,造成树冠管理困难,增加果实机械伤害,给果实采收也带来不便,对生产不利。但是,目前柑橘针刺发育和调控的分子机制尚不清楚,也无相关报道。本研究以‘枸头’橙(Citrus aurantium L.cv.'Goutou'Cheng)为研究材料,利用高通量测序平台Illumina HiSeq X Ten对针刺和枝进行了转录组测序。测序数据初步分析后共筛选到5441个差异表达基因,其中包含上调表达基因2881个、下调表达基因2560个。GO富集结果表明差异表达基因主要集中在生物过程(3170个基因)、代谢过程(2396个基因)、单生物过程(1742个基因)、氧化还原酶活性(520个基因)和结合活性(359个基因)等。差异表达基因KEGG代谢通路富集分析表明,富集程度比较显著的通路包括:次生代谢物生物合成(271个基因)、植物激素信号转导(73个基因)和苯丙素生物合成(55个基因)路径。其中,KEGG代谢通路富集分析结果表明次生代谢物合成的差异可能是针刺和枝的发育不同的重要因素,也暗示着次生代谢物合成路径可能在针刺发育过程起关键调控作用。本研究对枸头橙针刺和枝进行转录组测序并比较分析,为后期筛选柑橘针刺发育调控的关键基因和深入研究其分子调控机制提供了科学基础。Most of the branches of citrus have thorns,which cause many disadvantages in production,such as introducing difficulties to manage the canopy,increasing the mechanical damage of fruits,and bringing inconvenience to the fruit harvest.However,the molecular mechanisms regulating thorn development of citrus are still unclear,and no relevant reports were found.We performed transcriptome sequencing of thorns and branches of’Goutou’sour orange(Citrus aurantium L.cv.’Goutou’Cheng)by next generation of high-throughput sequencing technology,and differentially expressed genes was screened by comparative analysis of the transcriptome sequences of thorn and branch.Results showed that 5441 differently expressed genes were selected,of which 2881 were up-regulated and 2560 were down regulated.Based on GO analysis,most genes were classified as the functions involving biological process(3170 genes),metabolic process(2396 genes),single organism process(1742 genes),oxidoreductase activity(520 genes)and binding activity(359 genes).KEGG analysis results indicated that the differentially expressed genes mainly participated in biosynthesis of secondary metabolites(271 genes),plant hormone signal transduction(73 genes)and phenylpropanoid biosynthesis(55 genes).The results of KEGG pathway analysis showed that most differentially expressed genes between thorn and branch concentrated in the biosynthesis of secondary metabolites pathway,which provides insight into the different development mechanisms between thorn and branch and their link to secondary metabolism.Thus,we presumed that different secondary metabolites may be the key control factors of the citrus thorn development.These studies will lay the foundation for screening the key genes and revealing the molecular mechanism of citrus thorn.

关 键 词：‘枸头’橙 针刺 枝 转录组分析 差异表达基因 

分 类 号：S666.4[农业科学—果树学]