巴夫杜氏藻rbcS基因启动子的克隆及序列分析  

Cloning and Sequence Analysis of Promoter Region of Dunaliella parva rbcS Gene

作　　者：尚常花[1,2] 朱顺妮 Shang Changhua;Zhu Shunni(Guangdong Key Laboratory of New and Renewable Energy Research and Development,Key Laboratory of Renewable Energy,Chinese Academy of Sciences,Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences,Guangzhou,510640;College of Life Science,Guangxi Normal University,Guilin,541006)

机构地区：[1]中国科学院广州能源研究所,中国科学院可再生能源重点实验室,广东省新能源和可再生能源研究开发与应用重点实验室,广州510640 [2]广西师范大学生命科学学院,桂林541006

出　　处：《分子植物育种》2020年第19期6322-6327,共6页Molecular Plant Breeding

基　　金：中国科学院可再生能源重点实验室开放基金项目(Y807kb1001);广州市科技计划项目(201804010155);广西研究生教育创新计划项目(JGY2019009)共同资助。

摘　　要：根据已经获得的巴夫杜氏藻(Dunaliella parva)1,5-二磷酸核酮糖羧化酶/加氧酶小亚基基因(ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene,rbcS)的部分启动子序列设计三条特异引物,用于扩增延伸的启动子序列。采用Genome Walking方法,以总DNA为模板克隆了巴夫杜氏藻rbcS基因的延伸启动子序列1466 bp,启动子总长1582 bp。通过PlantCARE分析1582 bp序列,检测出启动子的基本元件TATA-box和CAAT-box。另外,还包括多个胁迫诱导元件,如光诱导元件、赤霉素响应元件、低温诱导元件等。值得注意的是,在启动子中发现了两个可以提供高转录水平的元件5UTR Py-rich stretch。该序列的克隆与分析为进一步研究巴夫杜氏藻rbcS的表达调控提供数据依据。Three gene specific primers were designed according to the obtained partial promoter of rbcS(ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene)from Dunaliella parva to amplify the extended promoter sequence.A 1466 bp extended promoter from Dunaliella parva was obtained by Genome Walking method with genomic DNA as a template.The total length of the promoter was 1582 bp.The functional elements were analyzed by PlantCARE software.The D.parva rbcS promoter contained the basic elements such as TATA-Box and CAAT-box and stress induced elements:light responsive element,gibberellin responsive element,cis-acting element involved in low temperature responsiveness,etc.Notably,two 5 UTR Py-rich stretch elements were found,which conferred high transcription levels.Cloning and characterization of the promoter region of D.parva rbcS made substantial basis for further research on the mechanism of regulation and expression of D.parva rbcS.

关 键 词：巴夫杜氏藻(Dunaliella parva) rbcS基因 染色体步移 启动子分析 

分 类 号：Q943.2[生物学—植物学]