病毒抑制子HCPro诱导拟南芥中YUC1的表达  

Viral Suppressor HCPro Induce the Expression of YUC1 in Arabidopsis thaliana

作　　者：金太成[1] 郎晨婧 王悦 吴艳菊 孟大伟[1] 杨丽萍[1] Jin Taicheng;Lang Chenjing;Wang Yue;Wu Yanju;Meng Dawei;Yang Liping(School of Life Science,Jilin Nonnal University,Siping,136000)

机构地区：[1]吉林师范大学生命科学学院,四平136000

出　　处：《分子植物育种》2020年第19期6372-6376,共5页Molecular Plant Breeding

基　　金：吉林省教育厅科研项目(JJKH20191013KJ);国家青年科学基金项目(31301043)共同资助。

摘　　要：生长素在植物的生长发育过程中发挥重要作用,病毒侵染影响宿主生长素的积累。本研究揭示了病毒抑制子HCPro诱导生长素生物合成基因表达的分子机制。利用农杆菌介导的叶片侵染法在拟南芥(Arabidopsis thaliana)中瞬时表达了报告基因GUS和病毒抑制子HCPro。结果证实,HCPro能够诱导YUC1基因启动子DNA去甲基化而激活其基因表达,为病毒与宿主互作的分子机制研究提供帮助。Auxin plays an important role in plant growth and development.Viral infection affects host auxin accumulation.In this study,we revealed the molecule mechanism by which the viral suppressor HCPro activated the expression of auxin biosynthetic gene in plants.We transiently expressed the foreign gene GUS and the viral suppressor HCPro in Arabidopsis thaliana by using Agrobacterium-mediated%leaf-infection.The results confirmed that HCPro can induce the DNA demethylation of YUC1 gene promoter and activate the gene expression in plants,and provid a basis for study on the molecule mechanism of interaction between virus and host.

关 键 词：拟南芥(Arabidopsis thaliana) 叶片侵染法 生长素生物合成基因 

分 类 号：Q943.2[生物学—植物学]