热带油茶根腐病病原菌的分子鉴定  

Molecular Identification of Pathogens Causing Root Rot of Camellia oleifera in Tropical

作　　者：赵志祥[1] 严婉荣[1] 肖敏[1] 肖彤斌 雷菲 Zhao Zhixiang;Yan Wanrong;Xiao Min;Xiao Tongbin;Lei Fei(Scientific Observation and Experiment Station of Crop Pests in Haikou,Ministry of Agriculture,Hainan Key Laboratory for Control of Plant Diseases and Insect Pests,Institute of Plant Protection of Hainan Academy of Agricultural Sciences,Haikou,571100;Hainan Key Laboratory of Arable Land Conservation,Institute of Agro-Environment and Soil of Hainan Academy of Agricultural Sciences,Haikou,571100)

机构地区：[1]海南省农业科学院植物保护研究所,海南省植物病虫害防控重点实验室,农业农村部海口作物有害生物科学观测实验站,海口571100 [2]海南省农业科学院农业环境与土壤研究所,海南省耕地保育重点实验室,海口571100

出　　处：《分子植物育种》2020年第19期6433-6440,共8页Molecular Plant Breeding

基　　金：海南省重点研发计划项目(ZDYF2018093);海南省财政科技计划项目共同资助。

摘　　要：油茶根腐病是普遍危害油茶树的一种根部病害,已成为影响海南山茶油生产的主要病害之一。为弄清其病原种类及其多样性关系,进而找到有效的防治策略,提高海南油茶成林率,本研究对海南油茶根腐病的病原菌进行了形态、分子鉴定和进化发育分析。从海口云龙、五指山、通什、红山、琼中、白沙、文昌等地采集腐根病样,经组织分离、形态特征观察、ITS序列测定和分析,GenBank中BLAST同源性比对搜索,以及构建系统进化树,分析系统进化发育关系。明确了海南油茶根腐病的病原有两种,一种为层生镰刀菌(Fusarium proliferatum),一种为尖孢镰刀菌(Fusarium oxysporum),以层生镰刀菌为主。形态学上代表菌株可分为两类,产生紫红色色素和黄褐色色素。序列比对和系统进化分析发现产生紫红色色素的代表菌株与层生镰刀菌的多条序列相似性为100%,并聚在同一分支上;产生黄褐色色素的代表菌株尖孢镰刀菌多条序列相似性为100%,并聚在同一分支上。这是国内首次报道油茶根腐病由两种以上病原菌单独或复合侵染引起。研究结果对油茶抗病育种和致病机理的研究具有重要的指导作用。Root rot of Camellia oleifera is a common root disease which has become one of the main diseases affecting the production of Camellia oleifera oil in Hainan.In order to understand the pathogenic species and their diversity,and then to find out effective prevention strategies,increase the rate of forestation in Hainan,the root rot of the pathogen was initiated by the methods of morphological analysis,molecular identification and phylogenetic analysis root rot samples were collected from Yunlong,Wuzhi Mountain,Tongshi,Hongshan,Qiongzhong,Baisha,Wenchang,and so on.The pathogen of Camellia oleifera root rot disease was studied by routine tissue separation and purification,morphological identificat ion,DNA sequence determination and analysis of ITS.At the same time,methods of comparing BLAST in GenBank database,building the system evolutionary tree,and phylogenetic analysis were used in this research.Morphological identification,molecular identification,and subsequent phylogenetic analysis showed that there were two pathogens of Camellia oleifera root rot in Hainan,one was Fusarium oxysporum,the other was Fusarium proliferatum.And Fusarium proliferatum was the major pathogenic type.Morphology on the representative strains could be divided into two classes,purple pigment and brown pigment.Sequence alignment and phylogenetic analysis found that the purple pigment representative strains were100%sequence similarity with Fusarium proliferatum,and get together on the same branch;Brown pigment representative strains were 100%sequence similarity with Fusarium oxysporum,and get together on the same branch.This is the first report of Camellia oleifera root rot caused by two or more pathogens alone or combined infection.The results of this study will be helpful to the breeding of Camellia oleifera resistance and the research of its pathogenic mechanism.

关 键 词：油茶(Camellia oleifera) 根腐病 分子鉴定 系统发育分析 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]