加工型辣椒花药培养胚状体诱导与植株再生分析  

Analysis on Embryo Induction and Plant Regeneration of Processing Type Pepper by Anther Culture

作　　者：李怡斐[1] 张世才[1] 蒋晓英[2] 杨小苗 王春萍[2] 林清[2] 黄启中[1] 黄任中[1] Li Yifei;Zhang Shicai;Jiang Xiaoying;Yang Xiaomiao;Wang Chun ping;Lin Qing;Huang Qizhong;Huang Renzhong(Vegetable and Flower Research Institute,Chongqing Academy of Agricultural Sciences,Chongqing,401329;Chongqing Key Laboratory of Adversity Agriculture,Biotechnology Research Center,Chongqing Academy of Agricultural Sciences,Chongqing,401329)

机构地区：[1]重庆市农业科学院蔬菜花卉研究所,重庆401329 [2]重庆市农业科学院生物技术研究中心,逆境农业研究重庆市重点实验室,重庆401329

出　　处：《分子植物育种》2020年第19期6467-6473,共7页Molecular Plant Breeding

基　　金：国家重点研发计划课题(2017YFD0101903);重庆市科研机构绩效激励引导专项(cstc2018jxjl80024);国家现代农业产业技术体系建设专项资金项目(CARS-24-A-04)共同资助。

摘　　要：为了利用花药培养技术快速创制特异育种材料,本试验以13个加工型辣椒F1代为试材,研究不同基因型、植物生长物质、Ag NO3、活性炭和植株开花期对加工型辣椒花药培养的影响。结果表明,基因型是影响花药培养的关键因素,92.31%的供试基因型能够诱导出胚状体,胚状体诱导率在0.17%~25.31%。加工型辣椒花药培养胚状体诱导最适合的培养基为MS+NAA 0.01 mg/L+6-BA 0.05 mg/L+Ag NO34 mg/L+活性炭4 g/L,诱导率为22.92%;植株处于初花期的花蕾胚状体诱导率最高,为27.74%,因此这个时期最适合加工型辣椒花药培养。本研究为加工型辣椒人工创制材料奠定了基础,为加快新品种选育起推动作用。In order to rapidly create specific breeding materials by anther culture technology,thirteen F1 processing type pepper were used to study the effects of different genotypes,exogenous hormones,Ag NO3,activated carbon,plants flowering on the anther culture in Capsicum annuum L..The results showed that 92.31%of the tested materials can induce embryoids,and the induction rate of embryoids was 0.17%~25.31%.MS+NAA 0.01 mg/L+6-BA 0.05 mg/L+Ag NO34 mg/L+activated carbon 4 g/L was the most suitable medium for embryoid induction in the anther culture of processing type pepper,and the induction rate was 22.92%.At early flowering buds was the most suitable time for anther culture,and the embryoid induction rate was 27.74%.In this study,the more effective embryo induction of anther culture were established,it laid a foundation for the creation of processing type pepper materials and accelerated the process of breeding new varieties.

关 键 词：加工型辣椒(Capsicum annuum L.) 花药培养 胚状体 外源激素 基因型 

分 类 号：S641.3[农业科学—蔬菜学]