高效液相色谱法测定人血浆卡托普利浓度  

Determination of Captopril in Plasma by High-performance Liquid Chromatography

作　　者：陈妍[1] 龚培[1] 崔华东 李一石[1] 

机构地区：[1]中国协和医科大学阜外心血管病医院临床药理研究室,北京100037

出　　处：《中国临床药理学杂志》2000年第4期289-291,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：建立柱前衍生化高效液相色谱法测定人血浆卡托普利浓度。方法：分析柱为Ｗａｔｅｒｓ Ｎｏｖａ－ｐａｋ Ｃ１８。流动相为乙脯：双蒸水：磷酸（１０３： １９７： ０３）。 ＵＶ检测波长为２６０ｎｍ。用对溴苯甲酰溴化物（ｐ－ＢＰＢ）与血浆的卡托普利在常温下衍生化反应３０ｍｉｎ。经衍生化反应的血浆酸化后用乙酸乙酯提取，分离有机相并碱化之，弃去上层，再进行酸化提取后测定。内标物为苯甲酰基苯甲酸。结果：测定卡托普利的线性范围为 １０～５００ｍｇ ·Ｌ－１，最低检测浓度为５ｍｇ· Ｌ－１。日内精密度＜８．２７％，日间精密度＜７．７０％。方法回收率均＞９０％。结论：本方法精密、准确，适用于卡托普利的药代动力学研究，也可用于测定尿中卡托普利浓度。OBJECTIVE: A modified procedure has been established and validated for the determination of concentration of captopril in human plasma. METHODS: Benzoyl-benzoic acid was used as the internal standard. The mobile phase consist of CH3CN:H2O:H3PO4 (103:197:0.3) at a flow-rate of 1 ml·min-1. The mixture of captopril and p-BPB was kept for 30min at ambient temperature to obtain the additional products. The extracts of samples were chromatographed on a Nova-pah C18 reversed-phase column after three steps liquidliquid extraction and monitored by UV-detector at 260nm. RESULTS: The calibration curve was linear over the range of 10-500 ng·ml-1. The intra-day RSD was less than 7.70% and the inter-day RSD less than 8.27%. The recovery was 90. 1 %-98.9% and the limit of quantification was 5ng·ml-1. CONCLUSION : The method has good selectivity, sensitivity and reproducibility and has proved to be suitable for pharmacokinetic studies of captopril in humans.

关 键 词：高效液相色谱 卡托普利 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R972[医药卫生—药学]