大气颗粒物暴露对高脂高糖饮食大鼠心肌组织Nrf2信号通路分子和炎症因子的影响  

Effects of atmospheric particulates exposure on Nrf2 signaling pathway and inflammatory factors in myocardium of rats fed with high-fat and high-glucose diet

作　　者：卞晶晶 王新茹 刘超 刘肖 刘颖 杨洪蕊 曹福源 李清钊 佟俊旺 蒋守芳 BIAN Jing-jing;WANG Xin-ru;LIU Chao;LIU Xiao;LIU Ying;YANG Hong-rui;CAO Fu-yuan;LI Qing-zhao;TONG Jun-wang;JING Shou-fang(Hebei Coal Mine Health and Safety Laboratory,School of Public Health,North China University of Science and Technology,Tangshan,Hebei 063210,China;Laboratory Animal Center,North China University of Science and Technology,Tangshan,Hebei 063210,China)

机构地区：[1]华北理工大学公共卫生学院,河北省煤矿卫生与安全实验室,河北唐山063210 [2]华北理工大学实验动物中心,河北唐山063210

出　　处：《上海预防医学》2020年第8期636-640,共5页Shanghai Journal of Preventive Medicine

摘　　要：【目的】研究大气颗粒物暴露对高脂高糖饮食大鼠心肌组织与氧化应激相关的核转录因子E2相关因子2(Nrf2)信号通路关键分子和炎症因子表达的影响。【方法】将48只SD雄性大鼠随机分成对照组(CC组)、高脂高糖组(HC组)、大气颗粒物组(CP组)及高脂高糖大气颗粒物组(HP组),每组12只。采用独立通气笼盒(IVC)饲养大鼠,CC组和HC组饲养于装有过滤大气颗粒物装置的IVC,CP组和HP组饲养于拆除大气颗粒物过滤装置的IVC,且其内空气成分接近室外空气成分,染毒3个月和6个月后分别处死24只大鼠,取心肌组织。用RT-qPCR法和Western blot法分别测定Nrf2、血红素加氧酶1(HO-1)、醌氧化还原酶1(NQO1)、血管细胞黏附因子(VCAM-1)、单核细胞趋化蛋白1(MCP-1)mRNA和VCAM-1、MCP-1的蛋白表达水平。【结果】与CC组比,HC组、CP组和HP组的Nrf2、HO-1、NQO1、VCAM-1和MCP-1 mRNA表达水平和VCAM-1、MCP-1的蛋白表达水平升高(P<0.05);与HC组和CP组相比,HP组的Nrf2、HO-1、NQO1、VCAM-1、MCP-1 mRNA和VCAM-1、MCP-1的蛋白表达水平升高(P<0.05);与3个月相比,6个月CP组和HP组的Nrf2 mRNA水平降低(P<0.05)。【结论】大气颗粒物、高脂高糖饮食及其联合暴露可引起心肌组织炎症反应,激活Nrf2通路通过抗氧化作用达到保护心肌组织的目的。[Objective]To study the effect of atmospheric particulate exposure on the expression of key molecules of Nrf2 signaling pathway involved in oxidative stress and inflammatory response factors in myocardium of rats fed with high-fat and high-glucose diet.[Methods]A total of 48 SD male rats were randomly divided into control group(CC group),high-fat and high-glucose diet group(HC group),atmospheric particulates group(CP group)and atmospheric particulates plus high-fat and high-glucose diet group(HP group),with 12 rats in each group.Rats were fed in individual ventilated cages(IVC).The CC and HC groups were placed in IVCs equipped with the atmospheric particulate filter,however,the CP and HP groups without the atmospheric particulate filter to make the air composition similar to the outdoor.A total of 24 rats were sacrificed for acquiring myocardial tissue after 3 and 6 months of exposure.The mRNA expression of Nrf2,HO-1,NQO1,VCAM-1 and MCP-1 were measured using RT-qPCR and the protein expression of VCAM-1,MCP-1 detected using western blot.[Results]The mRNA expression levels of Nrf2,HO-1,NQO1,VCAM-1 and MCP-1 and the protein expression levels of VCAM-1 and MCP-1 in HC,CP and HP groups were higher than CC group(P<0.05).The mRNA expression levels of Nrf2,HO-1,NQO1,VCAM-1,MCP-1 and the protein expression levels of VCAM-1,MCP-1 in the HP group were higher than HC and CP groups(P<0.05).The mRNA expression levels of Nrf2 in CP and HP groups after 6 months of exposure were lower than that at 3 months(P<0.05).[Conclusion]The exposure of atmospheric particles,high-fat and high-glucose and their combination diets could cause myocardial tissue inflammatory responses,and activate Nrf2 signaling pathways to protect against myocardial damage.

关 键 词：大气颗粒物 高脂高糖饮食 Nrf2信号通路 炎症因子 

分 类 号：R144[医药卫生—公共卫生与预防医学]