黄芩苷对兔冠状动脉球囊损伤后炎症和内皮功能的影响  

Effects of baicalin on inflammation and endothelial function after balloon injury of coronary artery in rabbits

作　　者：刘新锋 关玲霞 白保强 李新春 袁心刚 Liu Xinfeng;Guan Lingxia;Bai Baoqiang;Li Xinchun;Yuan Xingang(Interventional Operating Room,Zhumadian Central Hospital Affiliated to Huanghuai University,Zhumadian 463000,China;Fuwai Central China Cardiovascular Hospital,Zhenghou 450008,China)

机构地区：[1]黄淮学院附属驻马店市中心医院介入手术室,463000 [2]阜外华中心血管病医院(河南省人民医院心脏中心),郑州450008

出　　处：《中华实验外科杂志》2020年第8期1488-1491,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察黄芩苷对兔冠状动脉球囊损伤后炎症和内皮功能的影响。方法2018年6月至2019年6月,将32只购自河南省实验动物中心新西兰大白兔随机分为假手术组、模型组、低剂量组和高剂量组,每组8只,各组分别行冠状动脉球囊导管植入术,假手术组不植入球囊导管。低剂量组和高剂量组分别经腹腔注射黄芩苷100、300 mg/kg,假手术组和模型组经腹腔注射等体积生理盐水。治疗7 d后采用酶联免疫吸附试验(ELISA)检测各组外周血清炎性因子[肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β和IL-6]和内皮功能指标[一氧化氮(NO)、内皮素(ET-1)和血管紧张素Ⅱ(AngⅡ)]表达水平,采用蛋白质印迹法(Western blot)分析各组冠脉细胞间黏附分子-1(ICAM-1)和血管细胞间黏附分子-1(VCAM-1)蛋白表达水平,计量资料比较采用单因素方差分析。结果与模型组[(298.30±19.92)、(145.60±8.91)、(318.43±15.79)μg/L]比较,低剂量组兔外周血清中TNF-α、IL-1β和IL-6水平[(204.19±17.48)、(96.76±6.91)、(218.44±12.09)μg/L]显著下降,差异有统计学意义(t=3.818、4.127、3.002,P<0.05)。与低剂量组比较,高剂量组兔外周血清中TNF-α、IL-1β和IL-6水平[(116.77±9.42)、(42.12±5.19)、(120.41±7.98)μg/L]显著下降,差异有统计学意义(t=3.102、3.200、2.819,P<0.05)。与模型组中ET-1、AngⅡ和NO水平[(120.44±10.15)、(98.41±8.95)μg/L、(14.55±3.09)pmol/L]比较,低剂量组兔外周血清中ET-1和AngⅡ水平[(98.42±8.25)、(76.09±7.09)μg/L]显著下降,而NO水平[(21.11±4.29)pmol/L]显著增加,差异有统计学意义(t=3.618、3.091、2.901,P<0.05)。与低剂量组比较,高剂量组兔外周血清中ET-1和AngⅡ水平[(68.11±7.32)、(40.27±5.33)μg/L]显著增加,而NO水平[(29.49±4.10)pmol/L]显著下降,差异有统计学意义(t=2.510、2.298、2.091,P<0.05)。与模型组(1.10±0.11、1.22±0.1)比较,低剂量组兔血管组织ICAM-1和VCAM-1表达水平(0.92±0.15、0.40±0.12)�Objective To investigate the effects of Baicalin on inflammation and endothelial function after balloon injury in rabbits.Methods Thirty two New Zealand rabbits from Henan experimental animal center during June 2018 to June 2019 were randomly divided into sham operation group,model group,low-dose group and high-dose group(eight/per group).Rabbits in low-dose group and high-dose group were injected intraperitoneally by 100 mg/kg and 300mg/kg Baicalin,respectively,and rabbits in sham operation group and model group were injected intraperitoneally by the same volume of normal saline.After 7 days of treatment,the expression levels of inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand IL-6]and endothelial function indexes[nitric oxide(NO),ET-1 and angiotensinⅡ(AngⅡ)]were detected by enzyme linked immunosorbent assay(ELISA).The expression levels of intercellular adhesion molecule-1(ICAM-1)and VCAM-1 in coronary artery were analyzed by Western blotting.One-way ANOVA was used to compare the data between groups,and P<0.05 was statistically significant.Results Compared with the model group[(298.30±19.92),(145.60±8.91),(318.43±15.79)μg/L],the levels of TNF-α,IL-1βand IL-6 in the low-dose group[(204.19±17.48),(96.76±6.91),(218.44±12.09)μg/L]significantly decreased in the low-dose group(t=3.818,P<0.05;t=4.127,P<0.05;t=3.002,P<0.05).Compared with the low-dose group,the levels of TNF-α,IL-1βand IL-6 in the high-dose group[(116.77±9.42),(42.12±5.19),(120.41±7.98)μg/L]significantly decreased in the high-dose group(t=3.102,3.200,2.819,P<0.05).Compared with the levels of ET-1,AngⅡand NO in the model group[(120.44±10.15),(98.41±8.95)μg/L,14.55±3.09)pmol/L],the levels of ET-1 and AngⅡin the low-dose group[(98.42±8.25),(76.09±7.09)μg/L]significantly decreased,while the levels of no[(21.11±4.29)pmol/L]significantly increased(t=3.102,3.200,2.819,P<0.05).Compared with the low-dose group,the levels of ET-1 and AngⅡin the high-dose group[(68.11±7.32),(40.27±5.33)μg/L]significan

关 键 词：黄芩苷 球囊损伤 炎症 内皮功能 

分 类 号：R285.5[医药卫生—中药学]