藏菖蒲挥发油对多房棘球蚴的体外杀伤活性  

Study on the Lethal activity of Essential Oil From Acorus calamus L.Against Protoscoleces in vitro

作　　者：李占强[1] 李润乐[1] 苏姗姗 芦殿香[1] 汤锋[1] 樊海宁[3] LI Zhanqiang;LI Runle;SU Shanshan;LU Dianxiang;TANG Feng;FAN Haining(Research Center for High Altitude Medicine,Key Laboratory of Ministry of Education for High Altitude Medicine,Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province(Qinghai-Utah Joint Research Key Lab for High Altitude Medicine),Qinghai University,Qinghai,Xining 810003;Xining Customs Technical Center,Key Laboratory of Food Safety Research In Qinghai Province,Qinghai Xining 810003;Department of Hepatopancreatobiliary Surgery,Key Laboratory for Hydatid Diseases of Qinghai Province,Affiliated Hospital of Qinghai University,Qinghai,Xining 810001)

机构地区：[1]青海大学高原医学研究中心,高原医学教育部重点实验室,青海省高原医学应用基础重点实验室(青海-犹他高原医学联合重点实验室),青海西宁810001 [2]西宁海关技术中心,青海省食品安全研究重点实验室,青海西宁810003 [3]青海大学附属医院肝胆胰外科,青海省包虫病重点实验室,青海西宁810001

出　　处：《中国高原医学与生物学杂志》2020年第3期188-191,共4页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省科技厅重大科技专项(项目编号:2016-SF-A5);国家自然科学基金地区项目(81860299);青海省农牧厅项目(NMSY-2017-09)。

摘　　要：目的明确藏菖蒲挥发油对多房棘球蚴的体外杀伤活性。方法采用水蒸气蒸馏法制备藏菖蒲挥发油。以不同浓度的挥发油与原头蚴共培养,观察藏菖蒲挥发油对原头蚴的活力、死亡率及形态的影响。结果浓度为10 mg/mL的藏菖蒲挥发油致原头蚴活力下降、形态特征模糊、活动性消失。浓度为1.25 mg/mL的藏菖蒲挥发油作用5 h后致原头蚴死亡,死亡率达94.1%。电镜扫描死亡原头蚴示:形态变化显著,形态皱缩,体表凹凸不平,体表微绒毛消失。结论藏菖蒲挥发油在体外对多房棘球蚴具有较强的杀伤活性。Objective To identify the lethal activity of essential oil from Acorus calamus L.against protocercaria(Echinococcus multilocularis)in vitro.Methods The essential oil from A.calamus L.was prepared by steam distillation.The protocercaria were co-cultured with essential oil at different concentrations in vitro,and the motility,mortality and structural changes of protocercaria were observed.Results After co-culture of essential oil from A.calamus L.with a concentration of 10 mg/mL in vitro,the motility of protoscoleces decreased and further disappeared,morphological characteristics of protoscoleces were blurred.The mortality rate of protocercaria was 94.1% after co-culture with essential oil at a concentration of 1.25 mg/mL for 5 h.Scanning electron microscope results showed that microvilli on protocercaria surface were disappeared,and the shape of protocercaria was shrunken and uneven.Conclusion Essential oil from A.calamus L.has stronger effect against protocercaria in vitro.

关 键 词：藏菖蒲 挥发油 多房棘球蚴 活性 

分 类 号：R978.69[医药卫生—药品]