Cdc42在七氟烷神经毒性反应大鼠海马组织中的表达及其意义  

Expression and Significance of Cdc42 in the Hippocampus Rat Tissue Caused by Sevoflurane Neuro-toxicity

作　　者：韩涛[1] 陈琴 HAN Tao;CHEN Qin(Hunan Provincial Maternal und Child Health Care Hospial,Hunan Changsha 410006)

机构地区：[1]湖南省妇幼保健院,湖南长沙410006

出　　处：《医学临床研究》2020年第9期1343-1346,共4页Journal of Clinical Research

摘　　要：[目的]探讨细胞分裂周期蛋白42(Cdc42)在七氟烷神经毒性反应大鼠海马组织中的表达及其意义。[方法]7日龄SD大鼠42只。随机分为三组,每组14只。A组:2%七氟烷+80%氧气维持麻醉2 h,一次;B组:2%七氟烷+80%氧气维持麻醉2 h.1次/日.连续3d;C组:吸入80%氧气维持2 h.模型建立后3 h断颈取大脑海马组织,采用Western Blot检测海马组织中Cdc42、半胱氨酸蛋白水解酶3(C-Csp3)的表达水平。在出生后的45-49 d进行水迷宫实验检测大鼠空间学习记忆能力的变化。[结果]与C组比较.A组Cdc42,Cl-Csp3蛋白表达差并无统计学意义(P>0.05);B组Cdc42,Cl-Csp3的表达明显增加(P<0.05)。与C组比较,A组在定位航行实验中逃避潜伏期及空间探索实验中目标象限百分比比较差异无统计学意义(P>0.05),而B组在定位航行实验中逃避潜伏期明显延长。空间探索实验中目标象限百分比减少(P<0.05)。[结论]多次吸入七氟烷可导致新生大鼠大脑产生了异常的神经细胞凋亡.空间学习记忆能力下降,这可能与Cdc42蛋白表达水平升高有关。[Objective]The aim of this study is to explore the expression and significance of Cdc42(cell divi-sion cyelin42)in the hippocampus tissue of rats with neurotoxicity of sevoflurane.[Methods]Forty-two Sprague Dawley(SD)rats of 7 days old were randomly divided into three groups(n=14):group A.group B and group C.Rats in group A were exposed to 2%sevoflurane and 80%oxygen inhalation once for 2 hours.Rats in group B were exposed to 2%sevoflurane and 80%oxygen inhalation 2 hours/day for three consecutive days.Rats in group C received only 80%oxygen inhalation for 2 hours.Rats were sacrificed at 3 hours after experimental models com-pleted and the hippocampus tissues were collected to measure the expression of Cdc42 and cleaved caspase3(cyste-ine proteolytic enzyme 3,Cl-Csp3)by western blot.Spatial learning and memory ability of adult rats were detected by Morris water maze(MWM)test at 45-49 days after birth.[Results]Compared to the group C.the expression of Cdc42 and Cl-Csp3 in the hippocampus of the group A had no significant difference(P>0.05),whereas the expression of Cdc42 and Cl-Csp3 in the hippocampus of group B increased significantly(P<0.05).In place navi-gation test.there was no significant difference of the escape latency between group C and group A(P>0.05).The escape latency of rats in the group B were significantly longer than that of C group(P<0.05).In probe trial test,the percent time in target quadrant in group A was not different from that of groupC(P>0.05).While the percent time in target quadrant in group B decreased(P<0.05).[Conclusion]Multiple inhalation of sevoflurane can induce neurotoxicity and memory dysfunction,which may be related to the increase in the expression of Cdc42.

关 键 词：氟烷 大鼠 海马/病理学 免疫组织化学 

分 类 号：R338.64[医药卫生—人体生理学]