山柰酚减轻高糖条件下人肾小球内皮细胞氧化应激及凋亡的作用及机制研究  

The effect and mechanism of kaempferol on reducing oxidative stress and apoptosis of human glomerular endothelial cells under high glucose conditions

作　　者：郭晓燕[1] 胡春艳 李方晓 周燚[1] 张汀[1] 付丽娜[1] 马亚琪 GUO Xiaoyan;HU Chunyan;LI Fangxiao;ZHOU Yan;ZHANG Ting;FU Lina;MA Yaqi(Department of Nephrology,General Hospital of Ningxia Medical University,Yinchuan,Ningxia 750004,China;Department of Nephrology,the First Affiliated Hospital of Henan University,Kaifeng,Henan 475000,China;Department of Nephrology,Kaifeng Central Hospital,Kaifeng,Henan 475000,China)

机构地区：[1]宁夏医科大学总医院肾脏内科,银川750004 [2]河南大学第一附属医院肾脏内科,河南开封475000 [3]河南省开封市中心医院肾病科,475000

出　　处：《重庆医学》2020年第19期3201-3204,3210,共5页Chongqing medicine

摘　　要：目的探讨山柰酚减轻高糖条件下人肾小球内皮细胞(HRGEC)氧化应激及凋亡的作用机制。方法培养HRGEC并分组,对照组用普通培养基处理、高糖组用高糖培养基处理、山柰酚组用含有50μmol/L山柰酚的高糖培养基处理;检测氧化应激产物丙二醛(MDA)、总抗氧化力(T-AOC)的含量、凋亡率、基因核因子-E2相关因子2(Nrf2)、血红素加氧酶-1(HO-1)、哺乳动物雷帕霉素靶蛋白(mTOR)、含半胱氨酸的天冬氨酸蛋白水解酶-3(caspase-3)、AMP激活的蛋白激酶(AMPK)的表达量。结果高糖组MDA含量、凋亡率、caspase-3的表达量高于对照组,T-AOC含量及Nrf2、HO-1、mTOR、p-AMPK的表达量低于对照组(P<0.05);山柰酚组MDA含量、凋亡率、caspase-3的表达量低于高糖组,T-AOC含量及Nrf2、HO-1、mTOR、p-AMPK的表达量高于高糖组(P<0.05)。结论山柰酚能减轻高糖条件下HRGEC氧化应激及凋亡,激活AMPK通路是介导这一作用的可能分子机制。Objective To explore the effect and mechanism of kaempferol in reducing oxidative stress and apoptosis of human glomerular endothelial cells(HRGEC)under high glucose conditions.Methods Cultivate HRGEC and divide into groups,the control group is treated with normal medium,the high-sugar group is treated with high-sugar medium,and the kaempferol group is treated with high-sugar medium containing 50μmol/L kaempferol.Detection of oxidative stress product malondialdehyde(MDA),total antioxidant capacity(T-AOC)content,apoptosis rate,gene nuclear factor-E2 related factor 2(Nrf2),heme oxygenase-1(HO-1)The expression levels of mammalian target of rapamycin(mTOR),cysteine-containing aspartate proteolytic enzyme-3(caspase-3),and AMP-activated protein kinase(AMPK).Results The MDA content,apoptosis rate,and caspase-3 expression in the high glucose group were higher than those in the control group,and the T-AOC content and the expression levels of Nrf2,HO-1,mTOR,and p-AMPK were lower than those in the control group(P<0.05),the content of MDA,apoptotic rate and the expression of caspase-3 in kaempferol group were lower than those in high glucose group,the content of T-AOC and the expression of Nrf2,HO-1,mTOR and p-AMPK were higher than those in the high glucose group(P<0.05).Conclusion Kaempferol can alleviate HRGEC oxidative stress and apoptosis under high glucose conditions.Activation of AMPK pathway is a possible molecular mechanism that mediates this effect.

关 键 词：肾小球内皮细胞 高糖 山柰酚 氧化应激 凋亡 AMPK通路 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]