蛋白毒素AP-Toxin诱导撑绿杂交竹抗梢枯病及其分泌物响应  

Induced resistance of Bambusa pervariabilis× Dendrocalamopsis grandis to Arthrinium phaeospermum

作　　者：何倩倩[1,2] 彭麒 宗绡卓 朱天辉 李姝江[1,3] HE Qianqian;PENG Qi;ZONG Xiaozhuo;ZHU Tianhui;LI Shujiang(College of Forestry,Sichuan Agricultural University,Chengdu 611130,China;Yucheng District Forestry Bureau in Ya'an City,Ya'an 625000,China;National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River,Chengdu 611130,China)

机构地区：[1]四川农业大学林学院,四川成都611130 [2]雅安市雨城区林业局,四川雅安625000 [3]长江上游森林资源保育与生态安全国家林业和草原局重点实验室,四川成都611130

出　　处：《南京林业大学学报（自然科学版）》2020年第5期199-208,共10页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金(31700568);四川省科技厅项目(2020YJ0400)。

摘　　要：【目的】以撑绿杂交竹梢枯病病原菌(Arthrinium phaeospermum)灭活的蛋白毒素AP-Toxin为诱导因子,对在其诱导下3个杂交竹品种分泌物特性与病情指数、诱抗效果相关性进行分析,为诱导抗病性在林木病害治理中的应用奠定理论基础。【方法】选择健康的1年生撑绿杂交竹3号(No.3)、6号(No.6)、8号(No.8),对其上部嫩枝按4种处理方式进行针刺接种,接种后在4个不同时期分别采样。采用修订后的索姆基(Sormgy)糖含量测定法、双缩脲法、碱液滴定法分别测定分泌物中糖、蛋白质和脂类含量,分析糖、蛋白质和脂类分泌物对灭活毒素诱导的响应。【结果】撑绿杂交竹在引入灭活蛋白毒素作为诱导因子后,其分泌物(蛋白质、可溶性多糖、总糖、脂类)含量增加,病情指数显著下降、诱抗效果显著增加,撑绿杂交竹抵抗病原菌侵染的能力增强。在3个品种中,诱导后病情指数No.6最低、动态变化最小,其次是No.3,No.8病情指数最高。相关性分析表明,蛋白质、可溶性糖、总糖和脂类含量与病情指数和抗性显著正相关,其中还原糖含量与抗性呈显著负相关。【结论】暗孢节菱孢菌灭活蛋白毒素诱导后能使撑绿杂交竹抗性增强,抵抗病原菌的侵染;且抗性越强的品种,诱抗效果越好。【Objective】 The inactivated protein toxin fromArthrinium phaeospermum(AP-Toxin) was used as the induction factor for the resistance of threeBambusa pervariabilis×Dendrocalamopsis grandisvarieties, and the correlations of secretory response with the disease index and resistance were analyzed.【Method】 The upper twigs of healthy one-yearoldB.pervariabilis×D.grandisvarieties of No.3, 6, and 8 were inoculated with AP-Toxin by puncturing.The twig samples were collected at four different time points after inoculation.Secretion contents including sugar, proteins, and lipids were determined using the sormy sugar content determination method, biuret method, and alkali droplet method, respectively, with slight modifications.Responses of sugar, protein, and lipid contents after induction with AP-Toxin were assessed.【Result】 After induction with Ap-Toxin, protein, soluble sugar, total sugar, and lipids contents were increased in secretions of allB.pervariabilis×D.grandisvarieties.Moreover, the disease index of all varieties increased significantly.The resistance ofB.pervariabilis×D.grandistoA.phaeospermuminfestation was enhanced.Among the three varieties, the disease index of No.6 was the lowest and its secretory response was the weakest, whereas the disease index of No.8 was the highest and its secretory response was the strongest.Correlation analyses showed that protein, soluble sugar, total sugar, and lipid contents were significantly and positively correlated with the disease index and resistance,while the reducing sugar content was significantly and negatively correlated with disease resistance.【Conclusion】The resistance ofB.pervariabilis×D.grandisvarieties toA.phaeospermumcould be enhanced using AP-Toxin as the induction factor.The stronger the resistance of the variety, the better the induction effect.Our results lay the theoretical foundation for the application of induced disease resistance for the management of forest pests.

关 键 词：蛋白毒素 诱导因子 撑绿杂交竹梢枯病 分泌物响应 暗孢节菱孢菌 

分 类 号：S763.11[农业科学—森林保护学]