脂酶催化下大豆粉末磷脂与全氢化大豆油酯交换反应的研究  

Transesterification of Powdered Soybean Phospholipids and Hydrogenated Soybean Oil Catalyzed by Lipase

作　　者：李红 尹学智 徐改改 申瑞玲 毕艳兰[2] 吕喜丽 Li Hong;Yin Xuezhi;Xu Gaigai;Shen Ruiling;Bi Yanlan;LüXili(School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450000;Grain College,Henan University of Technology,Zhengzhou 450000)

机构地区：[1]郑州轻工业大学食品与生物工程学院,郑州450000 [2]河南工业大学粮油食品学院,郑州450000

出　　处：《中国粮油学报》2020年第9期70-76,共7页Journal of the Chinese Cereals and Oils Association

基　　金：2017年度河南省农业攻关项目(172102110135)。

摘　　要：利用脂酶的特异性催化作用,研究了正己烷体系中大豆粉末磷脂与全氢化大豆油的酯交换反应。利用碘值和产率为指标,考察了酶的种类及用量、底物摩尔比、温度、时间等因素对酯交换反应的影响,通过单因素和正交实验优化了大豆粉末磷脂与全氢化大豆油酯交换反应条件。发现在25%磷脂酶A1(以磷脂质量为基准)催化下,摩尔比4∶1的全氢化大豆油和大豆粉末磷脂的正己烷溶液(磷脂浓度为0.20 g/mL),在50℃下反应24 h,得到产率为72.9%的改性磷脂。与原料磷脂相比,改性磷脂的碘值由89 gI/100 g降至52 gI/100 g,脂肪酸组成变化较大,硬脂酸含量约为原料磷脂的9倍,不饱和脂肪酸亚麻酸和亚油酸含量降低了约一半,实现了大豆粉末磷脂的结构修饰。The transesterification of powdered soybean phospholipids and hydrogenated soybean oil catalyzed by lipase in hexane were studied in this paper.The effects of different parameters,including variety and amount of lipase,substrate molar ratio of hydrogenated soybean oil to powdered soybean phospholipids,temperature and the reaction time,were evaluated by single factor method test according to the changes of iodine value and yield.Furthermore,the process parameters were optimized by orthogonal experiment.The optimal conditions were predicted as follows:reaction temperature 50℃,reaction time 24 hours,amount of phospholipase A125%(%m/m of phospholipids),substrate molar ratio 4∶1,soybean phospholipid concentration 0.20 g/mL,and the optimal actual yield 72.9%.The iodine value of the modified phospholipids was reduced to 52 g/100 g from 89 g/100 g compared to that of the powdered soybean phospholipids.Moreover,the contents of fatty acids of modified phospholipids changed dramatically,in which the content of stearic acid was 9 times of that of raw material and the contents of linolenic acid and linoleic acid fell to around a half.

关 键 词：大豆粉末磷脂 全氢化大豆油 脂酶 酯交换反应 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]