山东省HIV-1分离株env基因C2-V3区序列测定  

SEQUENCE ANALYSIS OF HIV-1 evn C2-V3 REGION FROM HIV-1 ISOLATES IN SHANGDONG PROVINCE

作　　者：吴虹[1] 傅继华[2] 王斌[3] 王秋波[1] 张艳丽[1] 

机构地区：[1]青岛大学医学院免疫学教研室,青岛266021 [2]山东省卫生防疫站 [3]青岛大学医学院分子病毒学重点实验室

出　　处：《青岛大学医学院学报》2000年第3期157-160,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省科委自然科学基金!资助项目 ( 973 14 4 3 2 )

摘　　要：1目的　了解山东省 1型人类免疫缺陷病毒 (HIV- 1)的分子流行病学特征。 2方法　应用套式聚合酶链反应扩增 8例 HIV- 1前病毒基因组的 env基因 C2 - V 3区 ,扩增产物纯化后与 p GEM- T载体连接 ,克隆于大肠杆菌中 ,提取阳性克隆的重组质粒进行测序 ,用 PHYL IP软件分析核苷酸及氨基酸序列。 3结果　 8株 HIV - 1毒株V3环顶端的四肽基序均为 GPGR,氨基酸变异不显著 ,各株间基因离散率为 1.0 %～ 5 .2 % ,侧翼区碱基的变异频率明显高于 V3区 ,并发现 1例毒株出现双 V 3环现象。 4结论　山东省的 HIV - 1分离株以 B亚型 HIV- 1毒株为主 ,同时发现的双 V3环变异现象 ,可能是 HIV - 1毒株逃避宿主免疫系统攻击的一种新模式。Objective To investigate the molecular epidemiological features of HIV 1 strains in Shandong province at present time.\ Methods\ A 288 base pair fragment of env C2 V3 region was amplified by nested polymerase chain reaction(PCR) with genomic DNA extracted from PBMC as templates. The PCR purified products were connected with pGEM T vector then cloned into E.coli JM109. The recombinant plasmid DNA extracted from the positive clone were sequenced and analysed by PHILIP software.\ Results\ Our findings showed that all of the 8 isolates contained the GPGR tetrapeptide motif, the amino acid sequence variation was not significant and at the transition occurred randomly, the overall nucleotide sequence divergence ranged from 1.0%-5.2%. the variation of the sequences at the flanking of the V3 loop was higher than that of the V3 loop. Moreover, a special variation of double V3 loop was found in a individual sample.\ Conclusion\ The results demonstrate that subtype B is the predominant genetic subtype of the HIV 1 in Shandong, and the variation of double V3 loop implies a new way that the virus escapes from immune surveillance. [

关 键 词：山东 HIV-1分离株 ENV基因 C2-V3区 基因序列分析 分子流行病学 艾滋病 套式聚合酶链反应 

分 类 号：R373.9[医药卫生—病原生物学]