绵羊肝脏、肺脏细粒棘球蚴原头节差异蛋白质表达分析  

Differential protein expression analysis on protoscolex of Echinococcus granulosus in the liver and lung of sheep

作　　者：彭文军 曹得萍 张耀刚 刘燕[4] 姜博璠 赵海龙[4] PENG Wen-jun;CAO De-ping;ZHANG Yao-gang;LIU Yan;JIANG Bo-fan;ZHAO Hai-long(The Department of Animal Surgery of Qinghai University Medical College,Xining 810001,China;The Department of Human Parasitology of Bascic Medical College of Guilin Medical College,Guilin 541104,China;The Echinococcosis Key Laboratory of Qinghai University,Xining 810001,China;The Department of Pathogenic Biology of Qinghai University Medical College,Xining 810001,China)

机构地区：[1]青海大学医学院动物外科教研室,西宁810001 [2]桂林医学院基础学院人体寄生虫学教研室,桂林541104 [3]青海大学包虫病重点实验室,西宁810001 [4]青海大学医学院病原生物学教研室,西宁810001

出　　处：《中国人兽共患病学报》2020年第10期807-812,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金地区项目(No.81360255);青海省科技厅重大专项(No.2016-SF-A5);桂林医学院博士基金(No.20501019031)联合资助。

摘　　要：目的探讨绵羊肝脏和肺脏细粒棘球蚴原头节差异蛋白质表达谱,为虫体生长、发育调控特异性蛋白筛选奠定基础。方法提取寄生于绵羊肝脏与肺脏棘球蚴原头节蛋白质样本进行SDS-PAGE分析后,利用iTRAQ和液相色谱法以及串联质谱分析(LC-MS/MS)寄生于绵羊肝脏和肺脏细粒棘球蚴原头节蛋白质表达谱,通过Mascot软件和GO软件分析寄生于绵羊肝脏和肺脏棘球蚴原头节的差异表达蛋白质。结果本研究共鉴定了1229个有意义的蛋白质点,通过肝脏与肺脏比较有无差异性(肝脏原头节VS肺脏原头节)和调节趋势后获得217个差异表达蛋白质,在肝脏上调蛋白74个(脂肪酸绑定蛋白、ɑ纤维蛋白原、环氧化物酶、过氧化氢酶、酰基COA合成酶、载脂蛋白A4、磷酸化酶、6-磷酸葡萄糖内脂酶、乙酰辅酶酰基转移酶、还有一些未鉴定的蛋白等);下调蛋白143个(乳酸脱氢酶、谷胱甘肽过氧化物酶、内质网氧化还原酶、肌动蛋白、肌球蛋白、天冬酰胺酶、铁蛋白、6-磷酸葡萄糖酸脱氢酶、热休克蛋白90、胶原纤维I,胶原纤维IV、波形蛋白、玻连蛋白、磷脂转运蛋白、磷酸甘油酸激酶)。结论本研究发现寄生于绵羊肝脏和肺脏棘球蚴原头节蛋白质表达存在差异。To study the differential protein expression profile of the protoscolex of Echinococcus granulosus in liver and lung of sheep.In order to screening specific proteins for development and growth regulation of protoscolex.The protein was extracted from protoscolex in liver and lung of sheep from different groups and analyzed the protein profile by SDS-PAGE.And then the protein expression profiles of protoscolex between liver and lung were analyzed by iTRAQ and 2D liquid chromatography-tandem mass spectrometry(LC-MS).The differentially expressed proteins were analyzed by Mascot and GO software.In this study,a total of 1229 meaningful protein spots were identified.Differentially expressed proteins total 217 were obtained to compare Hepatic_P with Lung_P and the trend of regulation,of which 74 were up-regulated(eg,Fatty acid binding protein,Fibrinogen alpha,Epodide hydrolase,catalase,avyl-COA sythetase,apolipoprotein A4,glycogen phosphorylase,6-phosphogluconolactonase,acetyl-coenzyme acylthransferse and some uncharacterized proteins,and so on)and 143 were down-regulated(eg,L-lactate dehydrogenase,glutathione peroxidase,endoplasmic reticulum oxidoreductase,actin protein,myosin protein,asparaginase,ferritin,HSP90,6-phosphoglucoate dehydrogenase,I collagen,IV collagen,vimentin,vitronectin,phospholipid transfer protein,phosphoglycerate kinase and so on).In conclusion,it was found that the protein expression of the protoscolexof E.granulosus was different in sheep liver and lung.

关 键 词：细粒棘球绦虫 原头节 蛋白质组学 iTRAQ技术 二维液相色谱串联质谱 

分 类 号：R383.3[医药卫生—医学寄生虫学]