人工诱变利福平抗性布鲁氏菌转录组测序分析  

Transcriptional analysis of artificially induced rifampicin-resistant Brucella melitensis

作　　者：杨晓雯 赵鸿雁[1] 朴东日[1] 田国忠[1] 姜海[1] YANG Xiao-wen;ZHAO Hong-yan;PIAO Dong-ri;TIAN Guo-zhong;JIANG Hai(State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Bejing 102206,China)

机构地区：[1]传染病预防控制国家重点实验室,中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国人兽共患病学报》2020年第10期834-839,846,共7页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.81902105);侯云德院士科研青年基金项目(No.2019HYDQNJJ08)资助。

摘　　要：目的筛选布鲁氏菌中参与利福平代谢相关基因(除rpoB基因外)。方法利用人工诱变技术获得利福平抗性菌株,通过转录组测序获得标准菌株和利福平抗性菌株全基因组水平的基因表达量,利用EBSeq算法筛选差异表达基因,预测利福平代谢相关基因及主要代谢途径。结果通过不同浓度的利福平人工诱变,能够获得抗性表型稳定的布鲁氏菌变异菌株。转录组测序发现利福平抗性菌株中有121个基因表达量上调,197个基因表达量下调,差异基因功能主要集中于催化活性、细胞膜和细胞成分、代谢过程和细胞过程;主要参与抗生素的生物合成、细菌分泌系统和ABC转运蛋白等代谢通路。结论包括virB操纵子在内的涉及碳代谢等代谢通路的基因,通过表达量的改变参与抵抗利福平的作用。本研究为布鲁氏菌耐药相关基因的筛选提供新思路,同时为布鲁氏菌耐药菌株的防控提供基础性数据。The aim of this study was to screen rifampicin-resistance related genes(except for the rpoB gene).This study used artificial mutagenesis technology to obtain rifampin-resistant strains.And the gene expression levels of standard and rifampin-resistant strains at the whole genome level were obtained by transcriptome sequencing to predict rifampicin metabolism-related genes and major metabolic pathways in Brucella spp.The results showed that 121 genes were up-regulated and 197 genes were down-regulated in the rifampin-resistant strain.The functions of different expressed genes were mainly concentrated in catalytic activity,membrane part,cell,metabolic process and cellular process.They were mainly involved in metabolic pathways such as carbon metabolism,biosynthesis of antibiotics,bacterial secretion system,inositol phosphate metabolism and ABC transporters.This study found that genes including the virB operon participate in the resistance to rifampicin through changing in expression levels.This study provides new insights for the screening of Brucella resistance-related genes,and also provides basic data for the prevention and control of Brucella strains.

关 键 词：布鲁氏菌 利福平抗性 IV型分泌系统 RPOB 

分 类 号：R378.5[医药卫生—病原生物学]