HN蛋白糖基化位点缺失的新城疫病毒的生物学特性研究  

Biological Characteristics of a Newcastle Disease Virus Stain with Loss of Glycosylation Site of HN Protein

作　　者：艾惠 孙军峰[1] 陈琳娜 李乐 韩宗玺[1] 刘胜旺[1] AI Hui;SUN Jun-feng;CHEN Lin-na;LI Le;HAN Zong-xi;LIU Sheng-wang(Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China)

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150069

出　　处：《中国兽医杂志》2020年第6期1-4,共4页Chinese Journal of Veterinary Medicine

基　　金：“十二五”国家科技支撑计划课题(2015BAD12B03);国家重点研发计划(2018YFD0501302);国家自然科学基金(31872504)。

摘　　要：从黑龙江某鸡场中分离到1株新城疫病毒(NDV),命名为ck/CH/LHLJ170302株。全基因组序列测定和遗传进化分析表明,ck/CH/LHLJ170302株属于NDV class II类中的基因II型,与LaSota疫苗株的亲缘关系较近。分析基因分子特征发现,与LaSota株相比,ck/CH/LHLJ170302株编码的HN蛋白缺失了433位的糖基化修饰位点,而其他基因之间没有明显差异。进一步比较分析了ck/CH/LHLJ170302株与LaSota株在生长特性、致病性、抗原相关性方面的差异。结果发现,接种SPF鸡胚后,ck/CH/LHLJ170302株在24 h、48 h和72 h的病毒滴度均显著高于LaSota疫苗株(P<0.05),表明ck/CH/LHLJ170302株的增殖速度要快于LaSota株。毒力指标测定结果显示,ck/CH/LHLJ170302株引起鸡胚死亡平均时间(MDT)较LaSota株提前了近20 h,1日龄SPF雏鸡脑内接种致病指数(ICPI)略低于LaSota株。此外,ck/CH/LHLJ170302株与LaSota株的抗原相关性分析结果表明,ck/CH/LHLJ170302与LaSota株没有明显的抗原性差异。本研究结果为我国NDV的遗传变异的研究提供了参考数据。In this study,a strain of Newcastle disease virus(NDV)named ck/CH/LHLJ170302 was isolated from a chicken farm in Heilongjiang province.Whole-genome sequencing and genetic evolution analysis showed that the ck/CH/LHLJ170302 strain belonged to the genotype II of class II,and was closely related to the LaSota vaccine strain.Analysis of the molecular characteristics of the gene revealed that compared with LaSota strain,the HN protein encoded by the ck/CH/LHLJ170302 strain lost the N-linked glycosylation sites at aa433,and there was no significant difference among other genes.Furthermore,the differences in growth characteristics,pathogenicity,and antigen correlation between ck/CH/LHLJ170302 strain and LaSota vaccine strain were analyzed.The results showed that after inoculation with SPF chicken embryos,the virus titers of the ck/CH/LHLJ170302 strain at 24 h,48 h and 72 h were significantly higher than those of the LaSota vaccine strain,indicating that the ck/CH/LHLJ170302 strain grows faster than LaSota strain.The result of virulence index showed that the mean death time(MDT)in chicken embryo of ck/CH/LHLJ170302 was nearly 20 h earlier than that of the LaSota strain,and the intracebral pathogenicity index(ICPI)in 1-day-old SPF chicks of ck/CH/LHLJ170302 were lower than those of LaSota strain.In addition,the antigen correlation analysis between ck/CH/LHLJ170302 virus strain and LaSota vaccine strain showed that there was no significant antigen difference between ck/CH/LHLJ170302 and LaSota.The results of this study provide some insights into the research of NDV genetic variation in China.

关 键 词：新城疫病毒 HN蛋白 糖基化位点 生物学特性 

分 类 号：S852.65[农业科学—基础兽医学]