绵羊3种胞内寄生原虫感染情况调查  

Epidemiological Investigations of Three Intracellular Protozoan Parasites of Sheep

作　　者：陈雅婕 祝子伏 杨玉荣[2] 刘群 刘晶 CHEN Ya-jie;ZHU Zi-fu;YANG Yu-rong;LIU Qun;LIU Jing(College of Veterinary Medicine,China Agricultural University,National Animal Protozoa Laboratory,Key Laboratory of Animal Epidemiology of the Ministry of Agriculture,Beijing 100193,China;College of Animal Husbandry Engineering,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]中国农业大学动物医学院国家动物寄生原虫实验室农业部动物流行病学重点实验室,北京海淀100193 [2]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国兽医杂志》2020年第6期10-14,共5页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划(2017YFD0501304)。

摘　　要：为了解绵羊住肉孢子虫、新孢子虫和弓形虫流行情况,从河南省焦作市某绵羊饲养村,采集了114个心脏、61个食道肌和131份血清样品。应用镜检法对心脏和食道样本进行住肉孢子虫包囊检测,PCR扩增18S rRNA进行住肉孢子虫虫种鉴定;应用间接ELISA法检测新孢子虫和弓形虫血清抗体。结果显示,分离的住肉孢子虫均为柔嫩住肉孢子虫;住肉孢子虫阳性率为39.47%(45/114),新孢子虫血清阳性率为57.25%(76/131),弓形虫血清阳性率为21.37%(28/131),三者混合感染率为7.89%(9/114)。表明3种原虫感染在该绵羊群体中普遍流行,提示在绵羊饲养时要制定有效的防控措施。In order to investigate the epidemiology of Sarcocystis,Neospora caninum and Toxoplasma Gondii in sheep in Jiaozuo county,Henan Province,we collected 114 hearts,61 esophageal muscles and 131 serum samples from villages in Jiaozuo county.Cysts of Sarcocystis were detected by microscopic examination of the heart and esophagus samples.Purified bradyzoites were obtained by density gradient centrifugation.The 18S rRNA was amplified by PCR to identify the species of Sarcocystis.Antibodies to Neospora and Toxoplasma were detected by indirect ELISA.The results showed that Sarcocystis isolated from all samples were S.tenella,the prevalence of Sarcocystis was 39.47%(45/114),the prevalence of Neospora was 57.25%(76/131),the prevalence of Toxoplasma was 21.37%(28/131),and the mixed infection rate of the three was 7.89%(9/114).It showed that the three protozoa infections were prevalent in the sheep.The effective measures should be taken to prevent and control the three protozoa infections in sheep breeding.

关 键 词：住肉孢子虫 新孢子虫 弓形虫 血清阳性率 流行病学调查 

分 类 号：R531.5[医药卫生—内科学]